{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1306296000},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"34524.zip","Size":45893033,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"34524.jpg","Size":34386,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"34524.tif","Size":46000000,"Mime_type":"image\/tif"}],"CORE":{"HUMAN_DEV_ANATOMY":[{"onto_name":"gonadal component","onto_id":"EHDAA:4028"}],"ATTRIBUTION":{"DATE":["07\/2004"],"Contributors":["Michael Crammer"]},"BIOLOGICALPROCESS":[{"onto_name":"ciliary or flagellar motility","onto_id":"GO:0001539"},{"onto_name":"sperm motility","onto_id":"GO:0030317"}],"PROCESSINGHISTORY":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"CELLLINE":{"onto_name":"Mature sperm cell","onto_id":"FMA:67338"},"PARAMETERIMAGED":{"onto_name":"elastic scattering of photons","onto_id":"FBbi:00000587"},"IMAGEDESCRIPTION":{"free_text":"Human sperm diluted in buffer imaged at room temperature within two hours of ejaculation.  Cell at center swims at constant speed."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"flagellum","onto_id":"GO:0019861"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"differences in amount of elastic light scattering","onto_id":"FBbi:00000600"},"TECHNICALDETAILS":{"free_text":"Sperm were diluted with PBS and plated in a coverslip bottom chamber on an Olympus IX70 with 60X N.A. 1.4 phase 3 optics.  Images were collected with an air cooled CCD camera (either Roper Coolsnap or Cooke Sensicam) at 12 bits and converted to 8 bits.  The time interval was less than 1 second per image."},"DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"phase contrast microscopy","onto_id":"FBbi:00000247"},"DIMENSION":[{"Space":{"Image_size":640,"Pixel_size":{"unit":"microns","value":0.22},"axis":"X"}},{"Space":{"Image_size":480,"Pixel_size":{"unit":"microns","value":0.22},"axis":"Y"}}],"SPECIESTAXASPECIFIC":{"onto_id":"EHDAA:EHDAA:4028"},"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"},"CELLTYPE":{"onto_name":"sperm","onto_id":"CL:0000019"}}},"Citation":{"Title":"Michael Crammer (2011) CIL:34524, Homo sapiens, sperm. CIL. Dataset","ARK":"ark:\/b7295\/w9cil34524","DOI":"doi:10.7295\/W9CIL34524"}}}