CIL:12012, Hypsophrys nicaraguensis, keratocyte
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- Collection
- Cite This Work
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Yam, Patricia T.; Wilson, Cyrus A.; Ji, Lin; Hebert, Benedict; Barnhar, Erin L.; Dye, Natalie A.; Wiseman, Paul W.; Danuser, Gaudenz; Theriot, Julie (2021). CIL:12012, Hypsophrys nicaraguensis, keratocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0833QPR
- Description
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Spontaneous symmetry breaking and motility initiation of keratocytes. Phase-contrast time-lapse video of a keratocyte spontaneously breaking symmetry and initiating motility. The cell has been reoriented such that the eventual direction of movement is to the right. 50x real time (frames were collected at 5-s intervals and are displayed at 10 frames/s). Cells were imaged in culture media (Leibovitz's L-15 medium without phenol red supplemented with 14.2 mM Hepes, pH 7.4, 10% FBS, and 1% antibiotic-antimycotic). Images were acquired every 5 s. Corresponds to Fig 2A (cell A0 and video 3 in JCB 178:1207-1221, 2007.
- Date Issued
- 2021
- Researchers
- Technical Details
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Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Processing history: contrast, brightness, image combine
Data qualification: Raw;spatialmeasurements;intensitiesquantitation - Series
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 12012
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12012
- Yam PT, Wilson CA, Ji L, Hebert B, Barnhart EL, Dye NA, Wiseman PW, Danuser G, Theriot JA. Actin-myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility. J Cell Biol. 2007 Sep 24;178(7):1207-21. https://doi.org/10.1083/jcb.200706012
- Yam PT, Wilson CA, Ji L, Hebert B, Barnhart EL, Dye NA, Wiseman PW, Danuser G, Theriot JA. Actin-myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility. J Cell Biol. 2007 Sep 24;178(7):1207-21. https://www.ncbi.nlm.nih.gov/pubmed/?term=17893245
- Fawcett, Don W. "The Cell." 2nd ed., W.B. Saunders, 1981, ISBN 0721635849. https://www.ascb.org/career-development/teaching/don-fawcetts-the-cell/
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- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12