{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1294894800},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"12012.jpg","Size":21721,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Mp4","File_path":"12012_web.mp4","Size":1025151,"Mime_type":"video\/mp4"},{"File_type":"Zip","File_path":"12012.zip","Size":2449536,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":[" JCB 178:1207-1221, 2007"],"Contributors":["Patricia T. Yam","Cyrus A. Wilson","Lin Ji","Benedict Hebert","Erin L. Barnhar","Natalie A. Dye","Paul W. Wiseman","Gaudenz Danuser","Julie Theriot"],"PUBMED":["17893245"]},"BIOLOGICALPROCESS":[{"free_text":"symmetry breaking"},{"free_text":"motility initiation"}],"PROCESSINGHISTORY":{"free_text":"contrast, brightness, image combine"},"PARAMETERIMAGED":{"onto_name":"elastic scattering of photons","onto_id":"FBbi:00000587"},"IMAGEDESCRIPTION":{"free_text":"Spontaneous symmetry breaking and motility initiation of keratocytes. Phase-contrast time-lapse video of a keratocyte spontaneously breaking symmetry and initiating motility. The cell has been reoriented such that the eventual direction of movement is to the right. 50x real time (frames were collected at 5-s intervals and are displayed at 10 frames\/s). Cells were imaged in culture media (Leibovitz's L-15 medium without phenol red supplemented with 14.2 mM Hepes, pH 7.4, 10% FBS, and 1% antibiotic-antimycotic).  Images were acquired every 5 s.  Corresponds to Fig 2A (cell A0 and video 3 in JCB 178:1207-1221, 2007."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"actin cytoskeleton","onto_id":"GO:0015629"},"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"differences in amount of elastic light scattering","onto_id":"FBbi:00000600"},"GROUP_ID":"3422","DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"phase contrast microscopy","onto_id":"FBbi:00000247"},"DIMENSION":[{"Space":{"Image_size":327,"Pixel_size":{"unit":"microns","value":0.127},"axis":"X"}},{"Space":{"Image_size":310,"Pixel_size":{"unit":"microns","value":0.127},"axis":"Y"}},{"Time":{"unit":"seconds","value":5}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Hypsophrys nicaraguensis","onto_id":"NCBITaxon:131240"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"},"CELLTYPE":{"free_text":"keratocyte"}}},"Citation":{"Title":"Patricia T. Yam, Cyrus A. Wilson, Lin Ji, Benedict Hebert, Erin L. Barnhar, Natalie A. Dye, Paul W. Wiseman, Gaudenz Danuser, Julie Theriot (2011) CIL:12012, Hypsophrys nicaraguensis, keratocyte. CIL. Dataset","ARK":"ark:\/b7295\/w9cil12012","DOI":"doi:10.7295\/W9CIL12012"}}}