Apaja, Pirjo M.; Xu, Haijin; Lukacs, Gergely L. (2021). CIL:13681, Chlorocebus aethiops. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0P26WW1

To study the cellular consequences of misfolding of plasma membrane proteins, a transmembrane model protein that was constitutively targeted to the plasma membrane was developed for use as a reporter molecule. The reporter consisted of a C-terminally truncated CD4, incorporating a flexible cytoplasmic linker (CD4tl), fused to the N-terminal DNA-binding domain of the wild type (wt) bacteriophage lambda repressor (CD4tl-lambda) or L57C mutant repressor (CD4tl-lambdaC). The CD4tl-lambdaC cytosolic domain was largely in native state at 26°C but predominantly nonnative state at 37°C thus allowing the use of thermal shifts to follow the fate of unfolded proteins. In this image, one of six of a group from Figure 1 of Pirjo et al., JCB 2010, the expression of the mutant plasma membrane reporter, CD4tl-lambdaC, at 37 degrees is seen with CD4 antibody (green) in a detergent-permeabilized COS7 cell. The intracellular processing of this nonnative, thermally unfolded protein nearly completely limits its targeting to the plasma membrane. This cell is co-labeled with an ER marker, calreticulin antibody (red).

• 2021

• Apaja, Pirjo M.
• Lukacs, Gergely L.
• Xu, Haijin

Preparation: crosslinking-fixative fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Alexa Fluor 488; Alexa Fluor 555
Processing history: unprocessed raw data
Data qualification: Raw

• Cell Image Library Group ID: 7831

• Chlorocebus aethiops

• Endoplasmic reticulum
• Integral to plasma membrane

• COS-7
• Protein targeting to plasma membrane

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13681

Doi: https://doi.org/10.6075/J0P26WW1

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13681

Reference

• JCB 2010, 191:553-570 https://www.ncbi.nlm.nih.gov/pubmed/?term=20974815

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2022-08-12