{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1304136000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"13681.tif","Size":4300000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"13681.jpg","Size":154427,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"13681.zip","Size":4202775,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["JCB 2010, 191:553-570"],"Contributors":["Pirjo M. Apaja","Haijin Xu","Gergely L. Lukacs"],"PUBMED":["20974815"]},"BIOLOGICALPROCESS":{"onto_name":"protein targeting to plasma membrane","onto_id":"GO:0072661"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"COS-7","onto_id":"MCC:0000112"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"To study the cellular consequences of misfolding of plasma membrane proteins, a transmembrane model protein that was constitutively targeted to the plasma membrane was developed for use as a reporter molecule.  The reporter consisted of a C-terminally truncated CD4, incorporating a flexible cytoplasmic linker (CD4tl), fused to the N-terminal DNA-binding domain of the wild type (wt) bacteriophage lambda repressor (CD4tl-lambda) or L57C mutant repressor (CD4tl-lambdaC). The CD4tl-lambdaC cytosolic domain was largely in native state at 26°C but predominantly nonnative state at 37°C thus allowing the use of thermal shifts to follow the fate of  unfolded proteins.   In this image, one of six of a group from Figure 1 of Pirjo et al., JCB 2010, the expression of the mutant plasma membrane reporter, CD4tl-lambdaC, at 37 degrees is seen with CD4 antibody (green) in a detergent-permeabilized  COS7 cell.  The intracellular processing of this nonnative, thermally unfolded protein nearly completely limits its targeting to the plasma membrane.  This cell is co-labeled with an ER marker, calreticulin antibody (red)."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"integral to plasma membrane","onto_id":"GO:0005887"},{"onto_name":"endoplasmic reticulum","onto_id":"GO:0005783"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"7831","DATAQUALIFICATION":{"free_text":"RAW"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"DIMENSION":[{"Space":{"Image_size":1024,"Pixel_size":{"unit":"nanometers","value":47.8},"axis":"X"}},{"Space":{"Image_size":1024,"Pixel_size":{"unit":"nanometers","value":47.8},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Chlorocebus aethiops","onto_id":"NCBITaxon:9534"},"PREPARATION":[{"onto_name":"crosslinking-fixative fixed tissue","onto_id":"FBbi:00000008"},{"onto_name":"detergent permeabilized","onto_id":"FBbi:00000262"}],"VISUALIZATIONMETHODS":[{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"},{"onto_name":"Alexa Fluor 555","onto_id":"FBbi:00000443"}]}},"Citation":{"Title":"Pirjo M. Apaja, Haijin Xu, Gergely L. Lukacs (2011) CIL:13681, Chlorocebus aethiops. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13681","DOI":"doi:10.7295\/W9CIL13681"}}}