CIL:13754, Drosophila melanogaster, germ line cell, follicle cell, germ line stem cell, follicle stem cell
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- Collection
- Cite This Work
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Hartman, Tiffiney R.; Zinshteyn, Daniel; Schofield, Heather K.; Nicolas, Emmanuelle; Okada, Ami; O'Reilly, Alana M. (2021). CIL:13754, Drosophila melanogaster, germ line cell, follicle cell, germ line stem cell, follicle stem cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J01R6P71
- Description
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Hedgehog (Hh, red) is transcribed in apical cells of the Drosophila wild-type germarium (CIL# 13755, 13744). In the absence of boi (boi[e] mutant), Hh protein is redistributed from apical cells to the extracellular space of the local follicle stem cell niche (CIL# 13745), but Hh transcription is still in apical cells (this image; boi[e]; Hh-lacZ). Hh transcription is demonstrated by the Hh-lacZ reporter and follicle cells are highlighted using anti-Fas-3 (green). Image is Supplemental Figure S3B in J Cell Biol. 2010. 191: 943-952.
- Date Issued
- 2021
- Researchers
- Methods
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Fly ovaries were dissected and fixed as described previously (O'Reilly et al., 2008). Primary antibodies were anti-betagalactosidase and 1:25 mouse anti-Fas3 (Developmental Studies Hybridoma Bank [DSHB]. Secondary antibodies used were either FITC, Cy3, and/or Cy5 conjugated to species-specific secondary antibodies (Jackson ImmunoResearch Laboratories, Inc.). Samples were mounted in Vectashield mounting medium. Images were collected at room temperature (22C) using 40× (1.25 NA) or 63× (1.4 NA) oil immersion lenses (Leica) on an upright microscope (DM 5000; Leica) coupled to a confocal laser scanner (TCS SP5; Leica). LAS AF SP5 software (Leica) was used for data acquisition. Images representing individual channels of single confocal slices from the center of each germarium were exported as TIFF files, and images were converted to figures using Photoshop software (Adobe).
- Technical Details
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Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: primary antibody plus labeled secondary antibody; beta-galactosidase
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation - Series
- Scientific Name
- Anatomy
- Topics
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- Boi[e]
- Cell adhesion
- Cysteine-type endopeptidase activity
- Germ-line stem cell division
- Homophilic cell adhesion
- Morphogen activity
- Ovarian follicle cell development
- Patched binding
- Positive regulation of hh target transcription factor activity
- Positive regulation of smoothened signaling pathway
- Protein binding
- Smoothened signaling pathway
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 13754
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13754
- J Cell Biol. 2010. 191: 943-952. https://www.ncbi.nlm.nih.gov/pubmed/?term=21098113
- BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/
Source data
Reference
Other resource
- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12