{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1306900800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"13754.zip","Size":791897,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"13754.tif","Size":800000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"13754.jpg","Size":21562,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/3512"}],"PUBLISHED":["J Cell Biol. 2010. 191: 943-952."],"Contributors":["Tiffiney R. Hartman","Daniel Zinshteyn","Heather K. Schofield","Emmanuelle Nicolas","Ami Okada","Alana M. O'Reilly"],"PUBMED":["21098113"]},"BIOLOGICALPROCESS":[{"onto_name":"ovarian follicle cell development","onto_id":"GO:0030707"},{"onto_name":"homophilic cell adhesion","onto_id":"GO:0007156"},{"onto_name":"smoothened signaling pathway","onto_id":"GO:0007224"},{"onto_name":"cell adhesion","onto_id":"GO:0007155"},{"onto_name":"germ-line stem cell division","onto_id":"GO:0042078"},{"onto_name":"positive regulation of smoothened signaling pathway","onto_id":"GO:0045880"},{"onto_name":"positive regulation of hh target transcription factor activity","onto_id":"GO:0007228"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"free_text":"boi[e]"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Hedgehog (Hh, red) is transcribed in apical cells of the Drosophila wild-type germarium (CIL# 13755, 13744). In the absence of boi (boi[e] mutant), Hh protein is redistributed from apical cells to the extracellular space of the local follicle stem cell niche (CIL# 13745), but Hh transcription is still in apical cells (this image; boi[e]; Hh-lacZ). Hh transcription is demonstrated by the Hh-lacZ reporter and follicle cells are highlighted using anti-Fas-3 (green). Image is Supplemental Figure S3B in J Cell Biol. 2010. 191: 943-952."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"extracellular region","onto_id":"GO:0005576"},{"onto_name":"plasma membrane","onto_id":"GO:0005886"},{"onto_name":"cytoplasmic membrane-bounded vesicle","onto_id":"GO:0016023"},{"onto_name":"endocytic vesicle","onto_id":"GO:0030139"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"8382","MOLECULARFUNCTION":[{"onto_name":"morphogen activity","onto_id":"GO:0016015"},{"onto_name":"patched binding","onto_id":"GO:0005113"},{"onto_name":"cysteine-type endopeptidase activity","onto_id":"GO:0004197"},{"onto_name":"protein binding","onto_id":"GO:0005515"}],"TECHNICALDETAILS":{"free_text":"Fly ovaries were dissected and fixed as described previously (O\u2019Reilly et al., 2008). Primary antibodies were anti-betagalactosidase and 1:25 mouse anti-Fas3 (Developmental Studies Hybridoma Bank [DSHB]. Secondary antibodies used were either FITC, Cy3, and\/or Cy5 conjugated to species-specific secondary antibodies (Jackson ImmunoResearch Laboratories, Inc.). Samples were mounted in Vectashield mounting medium. Images were collected at room temperature (22C) using 40× (1.25 NA) or 63× (1.4 NA) oil immersion lenses (Leica) on an upright microscope (DM 5000; Leica) coupled to a confocal laser scanner (TCS SP5; Leica). LAS AF SP5 software (Leica) was used for data acquisition. Images representing individual channels of single confocal slices from the center of each germarium were exported as TIFF files, and images were converted to figures using Photoshop software (Adobe)."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"single-spot confocal microscopy","onto_id":"FBbi:00000252"},"DIMENSION":[{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.2365},"axis":"X"}},{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.2365},"axis":"Y"}},{"Space":{"Image_size":1,"Pixel_size":{"unit":"microns","value":0.042},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Drosophila melanogaster","onto_id":"NCBITaxon:7227"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"detergent permeabilized","onto_id":"FBbi:00000262"}],"VISUALIZATIONMETHODS":[{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"onto_name":"beta-galactosidase","onto_id":"FBbi:00000077"}],"CELLTYPE":[{"onto_name":"germ line cell","onto_id":"CL:0000039"},{"onto_name":"follicle cell","onto_id":"CL:0000477"},{"onto_name":"germ line stem cell","onto_id":"CL:0000014"},{"onto_name":"follicle stem cell","onto_id":"CL:0000441"}]}},"Citation":{"Title":"Tiffiney R. Hartman, Daniel Zinshteyn, Heather K. Schofield, Emmanuelle Nicolas, Ami Okada, Alana M. O'Reilly (2011) CIL:13754, Drosophila melanogaster, germ line cell, follicle cell, germ line stem cell, follicle stem cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13754","DOI":"doi:10.7295\/W9CIL13754"}}}