CIL:35166, Rattus rattus, permanent cell line cell
CIL source metadata (JSON)
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- Collection
- Cite This Work
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Bailly, Maryse; Cammer, Michael (2021). CIL:35166, Rattus rattus, permanent cell line cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0KH0M9C
- Description
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Constitutive expression of GFP (Green Fluorescent protein) in MTLn3 cells, a metastatic rat breast carcinoma line. This time lapse sequence of a single optical section near the substrate shows the cells jostling and the intrinsic movement of organelles that appear dark because they do not contain the fluorescent protein. This cell line is useful both as a cell culture system and also as a source of fluorescent cells that may be used to study metastasis in live rats or mice.
- Creation Date
- 2000-11-01
- Date Issued
- 2021
- Researchers
- Methods
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BioRad Radiance 2000 laser scanning confocal microscope with Kr/Ar laser for excitation of permanently transfected GFP at 488 nm.
The pixel size is approximate. - Technical Details
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Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: single-spot confocal microscopy
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation - Scientific Name
- Anatomy
- Topics
Formats
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 35166
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35166
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-08-17