{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1307246400},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"35166.zip","Size":10491004,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"35166.tif","Size":10600000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"35166.jpg","Size":79225,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"DATE":["11\/2000"],"Contributors":["Maryse Bailly","Michael Cammer"]},"BIOLOGICALPROCESS":{"onto_name":"interphase","onto_id":"GO:0051325"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"free_text":"MTLn3"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Constitutive expression of GFP (Green Fluorescent protein) in MTLn3 cells, a metastatic rat breast carcinoma line.  This time lapse sequence of a single optical section near the substrate shows the cells jostling and the intrinsic movement of organelles that appear dark because they do not contain the fluorescent protein.  This cell line is useful both as a cell culture system and also as a source of fluorescent cells that may be used to study metastasis in live rats or mice."},"ITEMTYPE":{"onto_name":"single-spot confocal microscopy","onto_id":"FBbi:00000252"},"CELLULARCOMPONENT":{"onto_name":"cytosol","onto_id":"GO:0005829"},"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"TECHNICALDETAILS":{"free_text":"BioRad Radiance 2000 laser scanning confocal microscope with Kr\/Ar laser for excitation of permanently transfected GFP at 488 nm.\n\n\nThe pixel size is approximate."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.16},"axis":"X"}},{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.16},"axis":"Y"}},{"Time":{"unit":"seconds","value":20,"frame":"40"}}],"SPECIESTAXASPECIFIC":{"onto_id":":null"},"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Rattus rattus","onto_id":"NCBITaxon:10117"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"EGFP","onto_id":"FBbi:00000082"},"CELLTYPE":{"onto_name":"permanent cell line cell","onto_id":"CL:0000002"},"PATHOLOGICALPROCESS":{"onto_id":":null"}}},"Citation":{"Title":"Maryse Bailly, Michael Cammer (2011) CIL:35166, Rattus rattus, permanent cell line cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil35166","DOI":"doi:10.7295\/W9CIL35166"}}}