Wadsworth, Patricia (2021). CIL:41466, Sus scrofa domestica, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0VM4B3D

Confocal image of a mitotic spindle in a dividing cell. The spindle is shown in yellow and the surrounding actin cytoskeleton is in blue. Images were acquired using a 100X NA 1.4 phase objective lens and a Perkin Elmer spinning disc confocal scan head. A Hammamatsu Orca ER cooled CCD camera was used to collect images. Image collection was driven using Metamorph software. Complete Z-series of images were collected and deconvolved using AutoQuant software. The image is a maximum intensity projection of a subset of the entire Z-stack; the same subset of the Z-stack is used in each channel. Sixth Prize, 2007 Olympus BioScapes Digital Imaging Competition.

• 2021

• Wadsworth, Patricia

Preparation: glutaraldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Visualization methods: phalloidin; primary antibody plus labeled secondary antibody; Fluorescein (FITC); Rhodamine
Processing history: deconvolution; maximum projection

• Sus scrofa domestica

• Actin cytoskeleton
• Epithelial cell
• Spindle

• LLC-PK1
• Mitosis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 41466

Doi: https://doi.org/10.6075/J0VM4B3D

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL41466

Other resource

• 2007 Olympus BioScapes Digital Imaging Competition®: https://www.olympus-lifescience.com/en/bioscapes/years/

Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12