{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1339732800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"41466.tif","Size":9300000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"41466.zip","Size":9300894,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"41466.jpg","Size":780175,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"OTHER":["University of Massachusetts Amherst, MA, USA"],"URLs":[{"Label":"2007 Olympus BioScapes Digital Imaging Competition","Href":"http:\/\/www.olympusbioscapes.com\/staticgallery\/2007\/6.html"}],"Contributors":["Patricia Wadsworth","2007 Olympus BioScapes Digital Imaging Competition"]},"BIOLOGICALPROCESS":{"onto_name":"mitosis","onto_id":"GO:0007067"},"PROCESSINGHISTORY":[{"free_text":"deconvolution"},{"free_text":"maximum projection"}],"CELLLINE":{"onto_name":"LLC-PK1","onto_id":"MCC:0000286"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Confocal image of a mitotic spindle in a dividing cell. The spindle is shown in yellow and the surrounding actin cytoskeleton is in blue.  Images were acquired using a 100X NA 1.4 phase objective lens and a Perkin Elmer spinning disc confocal scan head.  A Hammamatsu Orca ER cooled CCD camera was used to collect images. Image collection was driven using Metamorph software. Complete Z-series of images were collected and deconvolved using AutoQuant software.  The image is a maximum intensity projection of a subset of the entire Z-stack; the same subset of the Z-stack is used in each channel.  Sixth Prize, 2007 Olympus BioScapes Digital Imaging Competition."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"spindle","onto_id":"GO:0005819"},{"onto_name":"actin cytoskeleton","onto_id":"GO:0015629"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"},"TECHNICALDETAILS":{"free_text":"<img src=\"http:\/\/www.cellimagelibrary.org\/bioscapeslogo.png\" width=\"150\" height=\"56\">"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_nd"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"DIMENSION":[{"Space":{"Image_size":1908,"axis":"X"}},{"Space":{"Image_size":1620,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Sus scrofa domestica","onto_id":"NCBITaxon:9825"},"PREPARATION":{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},"VISUALIZATIONMETHODS":[{"onto_name":"phalloidin","onto_id":"FBbi:00000100"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"onto_name":"Fluorescein (FITC)","onto_id":"FBbi:00000451"},{"onto_name":"Rhodamine","onto_id":"FBbi:00000452"}],"CELLTYPE":{"onto_name":"epithelial cell","onto_id":"CL:0000066"}}},"Citation":{"Title":"Patricia Wadsworth, 2007 Olympus BioScapes Digital Imaging Competition (2012) CIL:41466, Sus scrofa domestica, epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil41466","DOI":"doi:10.7295\/W9CIL41466"}}}