Fawcett, Don W. (2021). CIL:10976, Myotis lucifugus, pancreatic acinar cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00K27CW

Transmission electron micrograph of nucleus showing the characteristic features of glutaraldehyde fixation introduced as a primary fixative in 1962 and since used routinely. Chromatin appears differentiated into coarse, darkly stained heterochromatin that accumulates near the nuclear periphery and the nucleolus and less darkly staining euchromatin. In contrast, chromatin appears uniformly stained with osmium tetroxide as primary fixative (for example, see CIL image 10974, in this image group). Figure 113 from Chapter 4 (Nucleus) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the corresponding chapter is available on the ASCB's BioEDUCATE website.

• 2021

• Fawcett, Don W.

Preparation: tissue in plastic embedment; osmium tetroxide fixed tissue; glutaraldehyde fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: film
Imaging mode: transmission electron microscopy (TEM); detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; osmium tetroxide; lead salt
Processing history: Image extracted from .pdf file of 'The Cell'
Data qualification: Processed

• Cell Image Library Group ID: 11224

• Myotis lucifugus

• Chromatin
• Nuclear envelope
• Nuclear heterochromatin
• Nucleolus
• Nucleus
• Pancreatic acinar cell
• Ribosome
• Rough endoplasmic reticulum

• Chromatin organization
• Nucleolus organization
• Nucleus organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 10976

Doi: https://doi.org/10.6075/J00K27CW

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL10976

Other resource

• Fawcett, Don W. "The Cell." 2nd ed., W.B. Saunders, 1981, ISBN 0721635849. https://www.ascb.org/career-development/teaching/don-fawcetts-the-cell/

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2022-08-12