Guervos, Marta (2021). CIL:40405, Convallaria majalis. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0W66JK3

Rendered 3D projection of a confocal z-series taken of rhizome (underground portion of the stem) of Convallari majalis (Lily of the Valley). The green objects represent reconstructed chloroplasts; cell walls are shown in red.

• 2021

• Guervos, Marta

Images were acquired with the Leica TCS AOBS SP2 confocal microscopy using a 63x oil immersion objective (NA 1.40) and an argon/krypton ion laser at wavelengths of 488 nm and an helium/neon 543 nm laser. The fluorescence signals were detected at 500-541 nm and 604-674 nm respectively. Z-series profiles of 30 optical sections were collected at intervals of 1.42 micrometers with a line average of 4 to reduce noise. Maximum projection of the series was applied. After confocal acquisition, z-stack was analysed with IMARIS software to adjust colours and to create 3D renders of chloroplasts.

Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of epitope
Processing history: 3D volume render

• Convallaria majalis

• Cell wall
• Chloroplast
• Rhizome

• Chloroplast localization
• Organelle organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 40405

Doi: https://doi.org/10.6075/J0W66JK3

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40405

Other resource

• Andor Insight Awards: https://andor.oxinst.com/

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

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2022-08-12