{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1331182800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"40405.tif","Size":4700000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"40405.jpg","Size":237715,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"OTHER":[" Image Processing Unit. University of Oviedo, Spain"],"URLs":[{"Label":"Andor Insight Awards","Href":"http:\/\/www.andor.com\/insight"},{"Label":"Andor Technology","Href":"http:\/\/purl.org\/NET\/andor.com"},{"Label":"Bitplane","Href":"http:\/\/www.bitplane.com"}],"Contributors":["Marta Guervos"]},"BIOLOGICALPROCESS":[{"onto_name":"chloroplast localization","onto_id":"GO:0019750"},{"onto_name":"organelle organization","onto_id":"GO:0006996"}],"PROCESSINGHISTORY":{"free_text":"3D volume render"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Rendered 3D projection of a confocal z-series taken of rhizome (underground portion of the stem) of Convallari majalis (Lily of the Valley).  The green objects represent reconstructed chloroplasts; cell walls are shown in red."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"chloroplast","onto_id":"GO:0009507"},{"onto_name":"cell wall","onto_id":"GO:0005618"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"},"PLANT_GROWTH":[{"free_text":"rhizome"}],"TECHNICALDETAILS":{"free_text":"Images were acquired with the Leica TCS AOBS SP2 confocal microscopy using a 63x oil immersion objective (NA 1.40) and an argon\/krypton ion laser at wavelengths of 488 nm and an helium\/neon 543 nm laser. The fluorescence signals were detected at 500-541 nm and 604-674 nm respectively. Z-series profiles of 30 optical sections were collected at intervals of 1.42 micrometers with a line average of 4 to reduce noise. Maximum projection of the series was applied. After confocal acquisition, z-stack was analysed with IMARIS software to adjust colours and to create 3D renders of chloroplasts."},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"DIMENSION":[{"Space":{"Image_size":1349,"axis":"X"}},{"Space":{"Image_size":1138,"axis":"Y"}}],"SPECIESTAXASPECIFIC":{"onto_id":"PO:rhizome"},"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Convallaria majalis","onto_id":"NCBITaxon:32189"}}},"Citation":{"Title":"Marta Guervos (2012) CIL:40405, Convallaria majalis. CIL. Dataset","ARK":"ark:\/b7295\/w9cil40405","DOI":"doi:10.7295\/W9CIL40405"}}}