Brunet, Stephane; Dumont, Julien; Lee, Karen W.; Kinoshita, Kazuhisha; Hikal, Pascale; Gruss, Oliver J.; Maro, Bernard; Verlhac, Marie-Helene (2021). CIL:35422, Mus musculus, oocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J07081FW

Time-lapse microscopy of mouse oocytes injected with a cRNA encoding YFP-TPX2 (targeting protein for Xenopus kinesin-like protein, xklp2), expressed at high levels. Up is the transmitted light and bottom corresponds to the YFP signal. Images were taken every 15 minutes. Times after the resumption of meiotic maturation and germinal vesicle breakdown (GVBD) are indicated in the lower left corner.

Video S2. Found at: doi:10.1371/journal.pone.0003338.s002

• 2021

• Brunet, Stephane
• Dumont, Julien
• Gruss, Oliver J.
• Hikal, Pascale
• Kinoshita, Kazuhisha
• Lee, Karen W.
• Maro, Bernard
• Verlhac, Marie-Helene

Time-lapse images were acquired using a Photometrics CCD camera (CoolSnap HQ2) mounted on a Leica DMI600B microscope using a Leica HC PL APO 20X/0.7 NA objective enclosed in a thermostatic chamber (Life Imaging Services). Immunofluorescence methods can be found in the cited paper and in Brunet S. et al. (1999) J Cell Biol 146: 1–11.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy; bright-field microscopy
Parameter imaged: fluorescence emission; absorption of illumination
Source of contrast: distribution of a specific protein; differences in adsorption or binding of stain
Visualization methods: Yellow fluorescent proteins from Aequorea; visualization of contiguous regions
Processing history: unprocessed raw data
Data qualification: Raw

• Cell Image Library Group ID: 8819

• Mus musculus

• Microtubule
• Microtubule organizing center part
• Nuclear chromosome
• Oocyte

• GTPase activity
• Meiotic spindle organization
• Microtubule organizing center organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 35422

Doi: https://doi.org/10.6075/J07081FW

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35422

Reference

• Brunet S, Dumont J, Lee KW, Kinoshita K, Hikal P, Gruss OJ, Maro B, Verlhac MH. Meiotic regulation of TPX2 protein levels governs cell cycle progression in mouse oocytes. PLoS One. 2008 Oct 3;3(10):e3338. https://doi.org/10.1371/journal.pone.0003338
• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=18833336

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2022-08-12