{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1311739200},"Data_type":{"Still_image":false,"Z_stack":false,"Video":false,"Time_series":true},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"35422.jpg","Size":9068,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"35422.zip","Size":6321767,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"35422.tif","Size":6400000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/www.plosone.org\/article\/info:doi%2F10.1371%2Fjournal.pone.0003338"}],"Contributors":["Stephane Brunet","Julien Dumont","Karen W. Lee","Kazuhisha Kinoshita","Pascale Hikal","Oliver J. Gruss","Bernard Maro","Marie-Helene Verlhac"],"PUBMED":["18833336"]},"BIOLOGICALPROCESS":[{"onto_name":"meiotic spindle organization","onto_id":"GO:0000212"},{"onto_name":"microtubule organizing center organization","onto_id":"GO:0031023"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":[{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},{"onto_name":"absorption of illumination","onto_id":"FBbi:00000308"}],"IMAGEDESCRIPTION":{"free_text":"Time-lapse microscopy of mouse oocytes injected with a cRNA encoding YFP-TPX2 (targeting protein for Xenopus kinesin-like protein, xklp2), expressed at high levels. Up is the transmitted light and bottom corresponds to the YFP signal. Images were taken every 15 minutes. Times after the resumption of meiotic maturation and germinal vesicle breakdown (GVBD) are indicated in the lower left corner.\n\nVideo S2. Found at: doi:10.1371\/journal.pone.0003338.s002"},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"microtubule","onto_id":"GO:0005874"},{"onto_name":"microtubule organizing center part","onto_id":"GO:0044450"},{"onto_name":"nuclear chromosome","onto_id":"GO:0000228"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":[{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"}],"GROUP_ID":"8819","MOLECULARFUNCTION":{"onto_name":"GTPase activity","onto_id":"GO:0003924"},"TECHNICALDETAILS":{"free_text":"Time-lapse images were acquired using a Photometrics CCD camera (CoolSnap HQ2) mounted on a Leica DMI600B microscope using a Leica HC PL APO 20X\/0.7 NA objective enclosed in a thermostatic chamber (Life Imaging Services). Immunofluorescence methods can be found in the cited paper and in Brunet S. et al. (1999) J Cell Biol 146: 1\u201311."},"DATAQUALIFICATION":{"free_text":"RAW"},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"IMAGINGMODE":[{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},{"onto_name":"bright-field microscopy","onto_id":"FBbi:00000243"}],"DIMENSION":[{"Space":{"Image_size":130,"axis":"X"}},{"Space":{"Image_size":260,"axis":"Y"}},{"Time":{"unit":"seconds","value":900,"frame":"62"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":[{"onto_name":"Yellow fluorescent proteins from Aequorea","onto_id":"FBbi:00000439"},{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"}],"CELLTYPE":{"onto_name":"oocyte","onto_id":"CL:0000023"}}},"Citation":{"Title":"Stephane Brunet, Julien Dumont, Karen W. Lee, Kazuhisha Kinoshita, Pascale Hikal, Oliver J. Gruss, Bernard Maro, Marie-Helene Verlhac (2011) CIL:35422, Mus musculus, oocyte. CIL. Dataset","ARK":"ark:\/b7295\/w9cil35422","DOI":"doi:10.7295\/W9CIL35422"}}}