Woodcock, Christopher (2021). CIL:448, Patiria miniata, sperm. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0WD3ZT4

Echinoderm sperm nuclei are unusual in that chromatin fibers are clearly seen, allowing their diameter to be accurately determined. Here, sperm from the starfish P. miniata were treated to remove the outer membrane, cryo-fixed by plunging into liquid ethane, freeze-substituted in acetone and low-temperature embedded in Lowicryl K11M. Thin sections were stained with uranyl acetate, and examined using a FEI CM10 TEM operated at 100KV. This image shows the three major components of the sperm cell, the crescent-shaped mitochondrion ad-pressed to the cup-shaped nucleus which surrounds the darkly-stained acrosome. Close-packed chromatin fibers are clearly seen in the nucleus

• 2021

• Woodcock, Christopher

Preparation: cryofixed tissue; tissue in plastic embedment; freeze substitution
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image; film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: intrinsic mass distribution; differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data

• Cell Image Library Group ID: 10230

• Patiria miniata

• Male germ cell nucleus
• Sperm

• Nucleus organization
• Reproduction
• Structural constituent of chromatin

• data
• image

• No linguistic content; Not applicable

Samplenumber: 448

Doi: https://doi.org/10.6075/J0WD3ZT4

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL448

Reference

• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=8138565
• Woodcock CL. Chromatin fibers observed in situ in frozen hydrated sections. Native fiber diameter is not correlated with nucleosome repeat length. J Cell Biol. 1994 Apr;125(1):11-9. https://doi.org/10.1083/jcb.125.1.11

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2022-08-12