{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1288065600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"448.zip","Size":21163884,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"448.jpg","Size":2772725,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"448.tif","Size":21000000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"Contributors":["Chris Woodcock"],"PUBMED":["8138565"]},"BIOLOGICALPROCESS":[{"onto_name":"reproduction","onto_id":"GO:0000003"},{"onto_name":"nucleus organization","onto_id":"GO:0006997"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Echinoderm sperm nuclei are unusual in that chromatin fibers are clearly seen, allowing their diameter to be accurately determined. Here, sperm from the starfish P. miniata were treated to remove the outer membrane, cryo-fixed by plunging into liquid ethane, freeze-substituted in acetone and low-temperature embedded in Lowicryl K11M. Thin sections were stained with uranyl acetate, and examined  using a FEI CM10 TEM operated at 100KV. This image shows the three major components of the sperm cell, the crescent-shaped mitochondrion ad-pressed to the cup-shaped nucleus which surrounds the darkly-stained acrosome. Close-packed chromatin fibers are clearly seen in the nucleus"},"ITEMTYPE":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"CELLULARCOMPONENT":{"onto_name":"male germ cell nucleus","onto_id":"GO:0001673"},"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":[{"onto_name":"intrinsic mass distribution","onto_id":"FBbi:00000607"},{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"}],"GROUP_ID":"10230","MOLECULARFUNCTION":{"onto_name":"structural constituent of chromatin","onto_id":"GO:0030527"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":4070,"Pixel_size":{"unit":"nanometers","value":0.72},"axis":"X"}},{"Space":{"Image_size":2576,"Pixel_size":{"unit":"nanometers","value":0.72},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Patiria miniata","onto_id":"NCBITaxon:46514"},"PREPARATION":[{"onto_name":"cryofixed tissue","onto_id":"FBbi:00000013"},{"free_text":"freeze substitution"},{"onto_name":"tissue in plastic embedment","onto_id":"FBbi:00000016"}],"VISUALIZATIONMETHODS":{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},"CELLTYPE":{"onto_name":"sperm","onto_id":"CL:0000019"}}},"Citation":{"Title":"Chris Woodcock (2010) CIL:448, Patiria miniata, sperm. CIL. Dataset","ARK":"ark:\/b7295\/w9cil448","DOI":"doi:10.7295\/W9CIL448"}}}