Thoma, Claudio R.; Matov, Alexandre; Gutbrodt, Katrin L.; Hoerner, Christian R.; Smole, Zlatko; Krek, Wilhelm; Danuser, Gaudenz (2021). CIL:26571, Homo sapiens, epithelial cell, renal cell carcinoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0B56HKC

Video shows the dynamics of microtubules (MTs) using a GFP fusion construct of EB3 (end binding protein 3) that transiently binds to growing MT plus ends, generating a punctate pattern of EB3-GFP comets throughout the cell. This video compares the number of EB3-GFP comets detected after nocodazole perturbation in an RCC-4 cell with active VHL. Compare with control video (RCC-4 alone) in CIL # 32024; there is an increased number of growing MTs when VHL is active. RCC-4 cells were infected with a retroviral vector to express EB3-GFP. Cells on round coverslips were transferred to a homemade holding device, and 600 µl of the appropriate medium, containing 40 nM nocodazole was added. The video was acquired with a microscope (IX70 Delta Vision Spectris; Olympus), temperature-controlled at 37C, using a 60× NA 1.4 differential interference contrast (DIC) oil Plan-Apochromat objective, ex 470 em 520, and a camera (CoolSNAP HQ; Roper Industries) with an exposure time of 100 ms and a frame rate of 0.5 s. Acquisition software used was SoftWoRx version 3.3.4 (Applied Precision). This is the raw image file for the right half of Video 5 in J Cell Biol. 2010. 190: 991-1003.

• 2021

• Danuser, Gaudenz
• Gutbrodt, Katrin L.
• Hoerner, Christian R.
• Krek, Wilhelm
• Matov, Alexandre
• Smole, Zlatko
• Thoma, Claudio R.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: widefield illumination; time lapse microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 8073

• Homo sapiens

• Cytoskeleton
• Epithelial cell
• Microtubule
• Microtubule plus end
• Renal cell carcinoma

• Protein binding
• Protein polymerization
• RCC4

• data
• image

• No linguistic content; Not applicable

Samplenumber: 26571

Doi: https://doi.org/10.6075/J0B56HKC

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL26571

Reference

• J Cell Biol. 2010. 190: 991-1003. https://www.ncbi.nlm.nih.gov/pubmed/?term=20855504

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

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2022-08-12