{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1305172800},"Data_type":{"Still_image":false,"Z_stack":false,"Video":false,"Time_series":true},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"26571.zip","Size":94490670,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"26571.tif","Size":94500000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"26571.jpg","Size":187878,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/3111\/"}],"PUBLISHED":["J Cell Biol. 2010. 190: 991-1003."],"Contributors":["Claudio R. Thoma","Alexandre Matov","Katrin L. Gutbrodt","Christian R. Hoerner","Zlatko Smole","Wilhelm Krek","Gaudenz Danuser"],"PUBMED":["20855504"]},"BIOLOGICALPROCESS":{"onto_name":"protein polymerization","onto_id":"GO:0051258"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"RCC4","onto_id":"MCC:0000395"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Video shows the dynamics of microtubules (MTs) using a GFP fusion construct of EB3 (end binding protein 3) that transiently binds to growing MT plus ends, generating a punctate pattern of EB3-GFP comets throughout the cell. This video compares the number of EB3-GFP comets detected after nocodazole perturbation in an RCC-4 cell with active VHL. Compare with control video (RCC-4 alone) in CIL # 32024; there is an increased number of growing MTs when VHL is active. RCC-4 cells were infected with a retroviral vector to express EB3-GFP. Cells on round coverslips were transferred to a homemade holding device, and 600 µl of the appropriate medium, containing 40 nM nocodazole was added. The video was acquired with a microscope (IX70 Delta Vision Spectris; Olympus), temperature-controlled at 37C, using a 60× NA 1.4 differential interference contrast (DIC) oil Plan-Apochromat objective, ex 470 em 520, and a camera (CoolSNAP HQ; Roper Industries) with an exposure time of 100 ms and a frame rate of 0.5 s. Acquisition software used was SoftWoRx version 3.3.4 (Applied Precision). This is the raw image file for the right half of Video 5 in  J Cell Biol. 2010. 190: 991-1003."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"microtubule","onto_id":"GO:0005874"},{"onto_name":"cytoskeleton","onto_id":"GO:0005856"},{"onto_name":"microtubule plus end","onto_id":"GO:0035371"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"8073","MOLECULARFUNCTION":{"onto_name":"protein binding","onto_id":"GO:0005515"},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"widefield illumination","onto_id":"FBbi:00000277"},{"onto_name":"time lapse microscopy","onto_id":"FBbi:00000249"}],"DIMENSION":[{"Space":{"Image_size":768,"Pixel_size":{"unit":"microns","value":0.1103},"axis":"X"}},{"Space":{"Image_size":768,"Pixel_size":{"unit":"microns","value":0.1103},"axis":"Y"}},{"Time":{"unit":"seconds","value":0.5,"frame":"80"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"EGFP","onto_id":"FBbi:00000082"},"CELLTYPE":[{"free_text":"renal cell carcinoma"},{"onto_name":"epithelial cell","onto_id":"CL:0000066"}]}},"Citation":{"Title":"Claudio R. Thoma, Alexandre Matov, Katrin L. Gutbrodt, Christian R. Hoerner, Zlatko Smole, Wilhelm Krek, Gaudenz Danuser (2011) CIL:26571, Homo sapiens, epithelial cell, renal cell carcinoma. CIL. Dataset","ARK":"ark:\/b7295\/w9cil26571","DOI":"doi:10.7295\/W9CIL26571"}}}