CIL:41000, Canis lupus familiaris, kidney cell
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- Collection
- Cite This Work
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Greb, Christoph; Jacob, Ralf; Schué, Anja (2021). CIL:41000, Canis lupus familiaris, kidney cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J06T0KD0
- Description
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Total internal reflection (TIRF) micrograph of MDCK cells stably transfected with the apical membrane protein P75-GFP (green) and Phalloidin staining of the actin cytoskeleton (red) in close proximity or inside the apical plasma membrane.
- Date Issued
- 2021
- Researchers
- Methods
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Cells were grown for 5 days prior to imaging with apical TIRF microscopy. Images were taken with a Leica AM TIRF MC. Penetration depth: 90 nm. See http://www.leica-microsystems.com/science-lab/protein-transport-processes-at-the-apical-membrane-of-polarized-epithelial-cells-applications-for-tirf-microscopy/ for additional details.
- Technical Details
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Preparation: formaldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: total internal reflection (TIRF)
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein; distribution of epitope
Visualization methods: EGFP; phalloidin
Processing history: unprocessed - Series
- Scientific Name
- Anatomy
- Topics
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 41000
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL41000
- Leica applications for TIRF microscopy ("protein transport processes at the apical membrane of polarized epithelial cells applications for tirf microscopy"): https://www.leica-microsystems.com/science-lab/science-lab-home/
Source data
Other resource
- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12