Greb, Christoph; Jacob, Ralf; Schué, Anja (2021). CIL:41000, Canis lupus familiaris, kidney cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J06T0KD0

Total internal reflection (TIRF) micrograph of MDCK cells stably transfected with the apical membrane protein P75-GFP (green) and Phalloidin staining of the actin cytoskeleton (red) in close proximity or inside the apical plasma membrane.

• 2021

• Greb, Christoph
• Jacob, Ralf
• Schué, Anja

Cells were grown for 5 days prior to imaging with apical TIRF microscopy. Images were taken with a Leica AM TIRF MC. Penetration depth: 90 nm. See http://www.leica-microsystems.com/science-lab/protein-transport-processes-at-the-apical-membrane-of-polarized-epithelial-cells-applications-for-tirf-microscopy/ for additional details.

Preparation: formaldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: total internal reflection (TIRF)
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein; distribution of epitope
Visualization methods: EGFP; phalloidin
Processing history: unprocessed

• Cell Image Library Group ID: 11683

• Canis lupus familiaris

• Actin filament
• Kidney cell
• P-75

• MDCK-II
• Protein targeting

• data
• image

• No linguistic content; Not applicable

Samplenumber: 41000

Doi: https://doi.org/10.6075/J06T0KD0

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL41000

Other resource

• Leica applications for TIRF microscopy ("protein transport processes at the apical membrane of polarized epithelial cells applications for tirf microscopy"): https://www.leica-microsystems.com/science-lab/science-lab-home/

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2022-08-12