{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1335758400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"41000.jpg","Size":26350,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"41000.tif","Size":300000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"Leica applications for TIRF microscopy","Href":"http:\/\/www.leica-microsystems.com\/science-lab\/protein-transport-processes-at-the-apical-membrane-of-polarized-epithelial-cells-applications-for-tirf-microscopy"}],"Contributors":["Christoph Greb","Ralf Jacob","Anja Schué"]},"BIOLOGICALPROCESS":{"onto_name":"protein targeting","onto_id":"GO:0006605"},"PROCESSINGHISTORY":{"free_text":"unprocessed"},"CELLLINE":{"onto_name":"MDCK-II","onto_id":"MCC:0000321"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Total internal reflection (TIRF) micrograph of MDCK cells stably transfected with the apical membrane protein P75-GFP (green) and Phalloidin staining of the actin cytoskeleton (red) in close proximity or inside the apical plasma membrane."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"free_text":"P-75"},{"onto_name":"actin filament","onto_id":"GO:0005884"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":[{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"}],"GROUP_ID":"11683","TECHNICALDETAILS":{"free_text":"Cells were grown for 5 days prior to imaging with apical TIRF microscopy. Images were taken with a Leica AM TIRF MC. Penetration depth: 90 nm.  See http:\/\/www.leica-microsystems.com\/science-lab\/protein-transport-processes-at-the-apical-membrane-of-polarized-epithelial-cells-applications-for-tirf-microscopy\/ for additional details."},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"free_text":"total internal reflection (TIRF)"},"DIMENSION":[{"Space":{"Image_size":261,"axis":"X"}},{"Space":{"Image_size":261,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Canis lupus familiaris","onto_id":"NCBITaxon:9615"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":[{"onto_name":"EGFP","onto_id":"FBbi:00000082"},{"onto_name":"phalloidin","onto_id":"FBbi:00000100"}],"CELLTYPE":{"onto_name":"kidney cell","onto_id":"CL:1000497"}}},"Citation":{"Title":"Christoph Greb, Ralf Jacob, Anja Schué (2012) CIL:41000, Canis lupus familiaris, kidney cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil41000","DOI":"doi:10.7295\/W9CIL41000"}}}