Vicente-Manzanares, Miguel; Koach, Margaret A.; Whitmore, Leanna; Lamers, Marcelo L. (2021). CIL:12410, Cricetulus griseus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0W66JJN

Localization and intracellular motility of myosin IIA (MIIA) in protrusive regions of migrating CHO.K1 cells. Arrowheads point to locations where representative MIIA filaments form. This Video corresponds to Fig. 6 A and video 3 in JCB 183:543-554 , 2008. Images of GFP-MIIA were collected every 4 s using TIRF microscopy. Images were acquired in an inverted microscope (model IX70;Olympus) with 488 nm excitation. A dichroic mirror (HQ485/30) was used for GFP-labeled cells. Images were acquired with a charge-coupled device camera (Retiga Exi; Qimaging) and analyzed using MetaMorph software (MDS Analytical Technologies). 10 frames/s shown.

• 2021

• Koach, Margaret A.
• Lamers, Marcelo L.
• Vicente-Manzanares, Miguel
• Whitmore, Leanna

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: TIRF
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 3714

• Cricetulus griseus

• Epithelial cell
• Myosin IIA

• CHO-K1
• Intracellular motility

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 12410

Doi: https://doi.org/10.6075/J0W66JJN

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12410

Reference

• JCB 183:543-554 , 2008 https://www.ncbi.nlm.nih.gov/pubmed/?term=18955554

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2022-08-12