{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1296190800},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Mp4","File_path":"12410_web.mp4","Size":513839,"Mime_type":"video\/mp4"},{"File_type":"Zip","File_path":"12410.zip","Size":3182720,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"12410.jpg","Size":41909,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["JCB 183:543-554 , 2008"],"Contributors":["Miguel Vicente-Manzanares","Margaret A. Koach","Leanna Whitmore","Marcelo L. Lamers"],"PUBMED":["18955554"]},"BIOLOGICALPROCESS":{"free_text":"intracellular motility"},"PROCESSINGHISTORY":{"free_text":"unprocessed raw data"},"CELLLINE":{"onto_name":"CHO-K1","onto_id":"MCC:0000103"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Localization and intracellular motility of myosin IIA (MIIA) in protrusive regions of migrating CHO.K1 cells. Arrowheads point to locations where representative MIIA filaments form.  This Video corresponds to Fig. 6 A and video 3 in JCB 183:543-554 , 2008. Images of GFP-MIIA were collected every 4 s using TIRF microscopy.  Images were acquired in an inverted microscope (model IX70;Olympus) with 488 nm excitation. A dichroic mirror (HQ485\/30) was used for GFP-labeled cells. Images were acquired with a charge-coupled device camera (Retiga Exi; Qimaging) and analyzed using MetaMorph software (MDS Analytical Technologies). 10 frames\/s shown."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"free_text":"myosin IIA"},"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"3714","DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"free_text":"TIRF"},"DIMENSION":[{"Space":{"Image_size":364,"Pixel_size":{"unit":"microns","value":0.076},"axis":"X"}},{"Space":{"Image_size":284,"Pixel_size":{"unit":"microns","value":0.076},"axis":"Y"}},{"Time":{"unit":"seconds","value":4}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Cricetulus griseus","onto_id":"NCBITaxon:10029"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"EGFP","onto_id":"FBbi:00000082"},"CELLTYPE":{"onto_name":"epithelial cell","onto_id":"CL:0000066"}}},"Citation":{"Title":"Miguel Vicente-Manzanares, Margaret A. Koach, Leanna Whitmore, Marcelo L. Lamers (2011) CIL:12410, Cricetulus griseus, epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil12410","DOI":"doi:10.7295\/W9CIL12410"}}}