Maresca, Thomas; Salmon, Edward (2021). CIL:25304, Drosophila melanogaster. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0125RDT

Confocal fluorescence time-lapse imaging of a mitotic Drosophila S2 cell expressing GFP-tubulin and treated with 100 nM taxol for 1 h before imaging to induce assembly of monopolar
spindles.
Images were recorded at 120 sec intervals using a spinning disc confocal microscope on a Nikon TE-200 stand with a 100x 1.4 NA Plan APO DIC objective lens and recorded with a Hamamatsu Orca-ER CCD. See Supplemental Video 5 in Maresca and Salmon, 2009 J Cell Biol 184:373-281.

• 2021

• Maresca, Thomas
• Salmon, Edward

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Green fluorescent proteins from Aequorea
Processing history: unprocessed raw data

• Drosophila melanogaster

• Spindle

• Mitosis
• S2

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 25304

Doi: https://doi.org/10.6075/J0125RDT

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL25304

Reference

• Maresca and Salmon, 2009 J Cell Biol 184:373-281 https://www.ncbi.nlm.nih.gov/pubmed/?term=19193623

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2022-08-12