{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1303099200},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"25304.jpg","Size":6201,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Mp4","File_path":"25304_web.mp4","Size":202418,"Mime_type":"video\/mp4"},{"File_type":"Zip","File_path":"25304.zip","Size":2588800,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["Maresca and Salmon, 2009 J Cell Biol 184:373-281"],"Contributors":["Thomas Maresca","Edward Salmon"],"PUBMED":["19193623"]},"BIOLOGICALPROCESS":{"onto_name":"mitosis","onto_id":"GO:0007067"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"free_text":"S2"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Confocal fluorescence time-lapse imaging of a mitotic Drosophila S2 cell expressing GFP-tubulin and treated with 100 nM taxol for 1 h before imaging to induce assembly of monopolar\nspindles. \nImages were recorded at 120 sec intervals using a spinning disc confocal microscope on a Nikon TE-200 stand with a 100x 1.4 NA Plan APO DIC objective lens and recorded with a Hamamatsu Orca-ER CCD. See Supplemental Video 5 in Maresca and Salmon, 2009 J Cell Biol 184:373-281."},"ITEMTYPE":{"onto_name":"charge coupled device (CCD)","onto_id":"FBbi:00000294"},"CELLULARCOMPONENT":{"onto_name":"spindle","onto_id":"GO:0005819"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},"DIMENSION":[{"Space":{"Image_size":248,"Pixel_size":{"unit":"microns","value":0.065},"axis":"X"}},{"Space":{"Image_size":248,"Pixel_size":{"unit":"microns","value":0.065},"axis":"Y"}},{"Time":{"unit":"seconds","value":120,"frame":"50"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Drosophila melanogaster","onto_id":"NCBITaxon:7227"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"Green fluorescent proteins from Aequorea","onto_id":"FBbi:00000437"}}},"Citation":{"Title":"Thomas Maresca, Edward Salmon (2011) CIL:25304, Drosophila melanogaster. CIL. Dataset","ARK":"ark:\/b7295\/w9cil25304","DOI":"doi:10.7295\/W9CIL25304"}}}