Ghosh, Rajarshi; Woodcock, Christopher (2021). CIL:1315, Mus musculus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J01Z43QK

The intracellular mobility of MeCP2 (a methylated DNA-binding protein) followed in a mouse fibroblast stably expressing GFP-MeCP2 through a time series of images captured by confocal microscopy after bleaching a 2 micrometer circular area of euchromatin (center, right). Fluorescence recovery after photobleaching (FRAP) is rapid, indicating that MeCP2 in euchromatin is highly mobile.

• 2021

• Ghosh, Rajarshi
• Woodcock, Christopher

Images were obtained using a Zeiss 510 Meta confocal microscope with excitation at 488 nm and emission between 505 nm and 530 nm. An initial scan of the cell was followed by the bleaching of a 2 um diameter spot of euchromatin, and 60 successive images recorded at 5 s intervals to measure fluorescence recovery due to MeCP2 mobility.

Preparation: whole mounted tissue
Relation to intact cell: living tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: fluorescence emission
Visualization methods: fluorescent proteins derived from Aequorea victoria
Processing history: unprocessed raw data

• Mus musculus

• Euchromatin
• Fibroblast
• Nuclear chromatin
• Nucleus

• Chromatin organization
• Nucleus organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 1315

Doi: https://doi.org/10.6075/J01Z43QK

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL1315

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12