{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1280980800},"Data_type":{"Still_image":false,"Z_stack":false,"Video":false,"Time_series":true},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"1315.zip","Size":19047095,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"1315.jpg","Size":57089,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"1315.tif","Size":16100000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"Contributors":["rajarshi ghosh",",\/chris woodcock"]},"BIOLOGICALPROCESS":[{"onto_name":"nucleus organization","onto_id":"GO:0006997"},{"onto_name":"chromatin organization","onto_id":"GO:0006325"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"The intracellular mobility of MeCP2 (a methylated DNA-binding protein) followed in a mouse fibroblast stably expressing GFP-MeCP2 through a time series of images captured by confocal microscopy after bleaching a 2 micrometer circular area of euchromatin (center, right). Fluorescence recovery after photobleaching (FRAP) is rapid, indicating that MeCP2 in euchromatin is highly mobile."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"nucleus","onto_id":"GO:0005634"},{"onto_name":"euchromatin","onto_id":"GO:0000791"},{"onto_name":"nuclear chromatin","onto_id":"GO:0000790"}],"RELATIONTOINTACTCELL":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"SOURCEOFCONTRAST":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"TECHNICALDETAILS":{"free_text":"Images  were obtained using a Zeiss 510 Meta confocal microscope with excitation at 488 nm and emission between 505 nm and 530 nm. An initial scan of the cell was followed by the bleaching of a 2 um diameter spot of euchromatin, and 60 successive images recorded at 5 s intervals to measure fluorescence recovery due to MeCP2 mobility."},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"single-spot confocal microscopy","onto_id":"FBbi:00000252"},"DIMENSION":[{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.04},"axis":"X"}},{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.04},"axis":"Y"}},{"Time":{"unit":"seconds","value":5,"frame":"61"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"VISUALIZATIONMETHODS":{"onto_name":"fluorescent proteins derived from Aequorea victoria","onto_id":"FBbi:00000420"},"CELLTYPE":{"onto_name":"fibroblast","onto_id":"CL:0000057"}}},"Citation":{"Title":"rajarshi ghosh, ,\/chris woodcock (2010) CIL:1315, Mus musculus, fibroblast. CIL. Dataset","ARK":"ark:\/b7295\/w9cil1315","DOI":"doi:10.7295\/W9CIL1315"}}}