Parysek, Linda; Aebig, Trudy (2021). CIL:248, Mus musculus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J06T0KW8

The distribution of mitochondria (labeled with antibody to heat shock protein 70) in relation to intermediate filaments (labeled with vimentin antibody) 16 hours after release from serum starvation in methanol-fixed 3T3 fibroblasts.

Microscope - Zeiss Axioplan I
Illumination - Mercury arc lamp
Objective - 100X oil/1.3 NA
Intermediate lens between obj and camera - 0.63X
Camera - Axiocam MRM CCD
Filters:
1. Ex 485/20, Em 515-565 BP, dichroic 510
2. Ex 560/40, Em 630/60, dichroic 595

• 2021

• Aebig, Trudy
• Parysek, Linda

Preparation: methanol fixed tissue
Relation to intact cell: whole mounted tissue
Item type: microscopy
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: localized accumulation of fluorescent probe
Visualization methods: Alexa Fluor 594; Alexa Fluor 488; primary antibody plus labeled secondary antibody
Processing history: unprocessed raw data

• Mus musculus

• Fibroblast
• Mitochondrion
• Type III intermediate filament

• ATP biosynthetic process
• Cytoplasm organization
• Intermediate filament-based process
• Mitochondrion morphogenesis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 248

Doi: https://doi.org/10.6075/J06T0KW8

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL248

CC0 1.0 Universal Public Domain Dedication

Creative Commons Public Domain Dedication

Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

Constraint(s) on Use: This work may be used without prior permission.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12