{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1285905600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"248.tif","Size":4100000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"248.jpg","Size":272832,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"248.zip","Size":4021478,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Linda Parysek","Trudy Aebig"]},"BIOLOGICALPROCESS":[{"onto_name":"intermediate filament-based process","onto_id":"GO:0045103"},{"onto_name":"mitochondrion morphogenesis","onto_id":"GO:0070584"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"The distribution of mitochondria (labeled with antibody to heat shock protein 70) in relation to intermediate filaments (labeled with vimentin antibody) 16 hours after release from serum starvation in methanol-fixed 3T3 fibroblasts.\n\nMicroscope - Zeiss Axioplan I\nIllumination - Mercury arc lamp\nObjective - 100X oil\/1.3 NA\nIntermediate lens between obj and camera - 0.63X\nCamera - Axiocam MRM CCD\nFilters:  \n1.  Ex 485\/20, Em 515-565 BP, dichroic 510\n2.  Ex 560\/40, Em 630\/60, dichroic 595"},"ITEMTYPE":{"onto_name":"microscopy","onto_id":"FBbi:00000241"},"CELLULARCOMPONENT":[{"onto_name":"type III intermediate filament","onto_id":"GO:0045098"},{"onto_name":"mitochondrion","onto_id":"GO:0005739"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"free_text":"localized accumulation of fluorescent probe"},"MOLECULARFUNCTION":[{"onto_name":"cytoplasm organization","onto_id":"GO:0007028"},{"onto_name":"ATP biosynthetic process","onto_id":"GO:0006754"}],"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":1300,"Pixel_size":{"unit":"nanometers","value":104},"axis":"X"}},{"Space":{"Image_size":1030,"Pixel_size":{"unit":"nanometers","value":104},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"methanol fixed tissue","onto_id":"FBbi:00000007"},"VISUALIZATIONMETHODS":[{"onto_name":"Alexa Fluor 594","onto_id":"FBbi:00000444"},{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"}],"CELLTYPE":{"onto_name":"fibroblast","onto_id":"CL:0000057"}}},"Citation":{"Title":"Linda Parysek, Trudy Aebig (2010) CIL:248, Mus musculus, fibroblast. CIL. Dataset","ARK":"ark:\/b7295\/w9cil248","DOI":"doi:10.7295\/W9CIL248"}}}