Ris, Hans (2021). CIL:35389, Drosophila melanogaster, salivary gland. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J01J98PZ

High Voltage (1MeV) TEM image of a 'thick' section of polytene chromosome from salivary gland of 3rd instar larva of Drosophila melanogaster showing the band-interband organization. This image was captured with a specimen tilt of 56 degrees. Grouped with it is an image of the same region taken at a tilt angle of 45 degrees, providing an oblique stereo view of the structure.

• 2021

• Ris, Hans

Salivary glands were dissected, fixed with paraformaldehyde, post-fixed with osmium tetroxide, and embedded in plastic. 4 micron sections were cut, stained with aqueous uranyl magnesium acetate, and observed with the Wisconsin HVEM at 1 MeV. See Ris, H 1981 Stereoscopic Electron Microscopy of chromosomes. Meth Cell Biol 22:77-96; Ris, H 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246

Preparation: chemically fixed tissue; osmium tetroxide fixed tissue; tissue in plastic embedment; paraformaldehyde fixation
Relation to intact cell: microtome-sectioned tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data

• Cell Image Library Group ID: 11163

• Drosophila melanogaster

• Polytene chromosome
• Salivary gland

• Chromosome organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 35389

Doi: https://doi.org/10.6075/J01J98PZ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35389

CC0 1.0 Universal Public Domain Dedication

Creative Commons Public Domain Dedication

Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

Constraint(s) on Use: This work may be used without prior permission.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12