{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1309147200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"35389.jpg","Size":5986531,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"35389.tif","Size":41300000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"35389.zip","Size":43763306,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Hans Ris, University of Wisconsin, Madison, WI"]},"BIOLOGICALPROCESS":{"onto_name":"chromosome organization","onto_id":"GO:0051276"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"High Voltage (1MeV) TEM image of a 'thick' section of polytene chromosome from salivary gland of 3rd instar larva of Drosophila melanogaster showing the band-interband organization. This image was captured with a specimen tilt of 56 degrees. Grouped with it is an image of the same region taken at a tilt angle of 45 degrees, providing an oblique stereo view of the structure."},"ITEMTYPE":{"onto_name":"film","onto_id":"FBbi:00000303"},"CELLULARCOMPONENT":{"onto_name":"polytene chromosome","onto_id":"GO:0005700"},"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"11163","TECHNICALDETAILS":{"free_text":"Salivary glands were dissected, fixed with paraformaldehyde, post-fixed with osmium tetroxide, and embedded in plastic. 4 micron sections were cut, stained with aqueous uranyl magnesium acetate, and observed with the Wisconsin HVEM at 1 MeV. See Ris, H 1981 Stereoscopic Electron Microscopy of chromosomes. Meth Cell Biol 22:77-96; Ris, H 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_id":"FBbi:00000622"},"DIMENSION":[{"Space":{"Image_size":5580,"Pixel_size":{"unit":"nanometers","value":1.5},"axis":"X"}},{"Space":{"Image_size":3690,"Pixel_size":{"unit":"nanometers","value":1.5},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Drosophila melanogaster","onto_id":"NCBITaxon:7227"},"PREPARATION":[{"onto_name":"chemically fixed tissue","onto_id":"FBbi:00000002"},{"free_text":"paraformaldehyde fixation"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in plastic embedment","onto_id":"FBbi:00000016"}],"VISUALIZATIONMETHODS":{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},"CELLTYPE":{"free_text":"salivary gland"}}},"Citation":{"Title":"Hans Ris, University of Wisconsin, Madison, WI (2011) CIL:35389, Drosophila melanogaster, salivary gland. CIL. Dataset","ARK":"ark:\/b7295\/w9cil35389","DOI":"doi:10.7295\/W9CIL35389"}}}