Svitkina, Tatyana M.; Borisy, Gary G. (2021). CIL:24786, Xenopus laevis, keratocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0G44P56

Multiple branching of actin filaments in lamellipodia of Xenopus keratocytes. This image shows an overview of the leading edge, and CIL 24787 shows enlargements of local regions of this platinum replica. CIL 24788 shows a similar overview for the lamellipodia of a vertebrate fibroblasts. Image corresponds to Figure 1a from J Cell Biol. 1999 May 31;145(5):1009-26.

• 2021

• Borisy, Gary G.
• Svitkina, Tatyana M.

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Preparation: permeabilized tissue; glutaraldehyde fixed tissue; phalloidin; polyethylene glycol
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: optical path length gradient
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating; platinum replica
Processing history: unprocessed raw data

• Cell Image Library Group ID: 9875

• Xenopus laevis

• Actin cytoskeleton
• Keratocyte
• Lamellipodium

• Actin filament-based process
• Branching of actin filaments

• data
• image

• No linguistic content; Not applicable

Samplenumber: 24786

Doi: https://doi.org/10.6075/J0G44P56

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL24786

Reference

• J Cell Biol. 1999 May 31;145(5):1009-26. https://www.ncbi.nlm.nih.gov/pubmed/?term=10352018

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2022-08-12