{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1307073600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"24786.zip","Size":10758194,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"24786.tif","Size":10800000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"24786.jpg","Size":1912934,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Biol. 1999 May 31;145(5):1009-26."],"Contributors":["Tatyana M. Svitkina","Gary G. Borisy"],"PUBMED":["10352018"]},"BIOLOGICALPROCESS":[{"free_text":"branching of actin filaments"},{"onto_name":"actin filament-based process","onto_id":"GO:0030029"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"optical path length gradient","onto_id":"FBbi:00000312"},"IMAGEDESCRIPTION":{"free_text":"Multiple branching of actin filaments in lamellipodia of Xenopus keratocytes. This image shows an overview of the leading edge, and CIL 24787 shows enlargements of local regions of this platinum replica.  CIL 24788 shows a similar overview for the lamellipodia of a vertebrate fibroblasts. Image corresponds to Figure 1a from J Cell Biol. 1999 May 31;145(5):1009-26."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"lamellipodium","onto_id":"GO:0030027"},{"onto_name":"actin cytoskeleton","onto_id":"GO:0015629"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"differences in deposition of metal shadow","onto_id":"FBbi:00000601"},"GROUP_ID":"9875","TECHNICALDETAILS":{"free_text":"Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998)."},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":3286,"axis":"X"}},{"Space":{"Image_size":3268,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Xenopus laevis","onto_id":"NCBITaxon:8355"},"PREPARATION":[{"onto_name":"permeabilized tissue","onto_id":"FBbi:00000093"},{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"free_text":"phalloidin"},{"free_text":"polyethylene glycol"}],"VISUALIZATIONMETHODS":[{"onto_name":"shadowing and plating","onto_id":"FBbi:00000398"},{"free_text":"platinum replica"}],"CELLTYPE":{"free_text":"keratocyte"}}},"Citation":{"Title":"Tatyana M. Svitkina, Gary G. Borisy (2011) CIL:24786, Xenopus laevis, keratocyte. CIL. Dataset","ARK":"ark:\/b7295\/w9cil24786","DOI":"doi:10.7295\/W9CIL24786"}}}