Roels, Frank (2021). CIL:48351, Sprague-Dawley rat. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J02B8Z5C

Images are acquired from fixed and living heart tissue using fiber-optics and laser-scanning confocal microscopy, respectively. Three sets of images denoted as CCM, FCMtopical, FCMcarrier were stored for subsequent analyses.

• 2021

• Roels, Frank

CCM images were from fixed rodent tissue preparations acquired using established methods (doi: 10.1161/circimaging.112.000121). In short, rodent hearts were isolated and Langendorff-perfused with 4% paraformaldehyde. Tissue from the atrial working myocardium, sinoatrial node, and atrioventricular node were dissected from the hearts and fluorescently labeled with wheat germ agglutinin conjugated to CF488A (29022-1; Biotium, Hayward, CA; 1:25). Fluorescently labeled tissue preparations were imaged using a conventional laser-scanning confocal microscope (Zeiss LSM5 Duo; Zeiss, Jena, Germany) with a 40x oil immersion lens (NA 1.3). The fluorescent signal was captured using an Argon/2 ion laser (488 nm excitation) and a bandpass filter (505 to 555 nm emission). Three-dimensional image stacks were acquired at a spatial resolution (xyz dimensions), field of view (xy) and a z-scan range of 0.2x0.2x0.2 μm, 204.8x204.8 μm and 50 μm, respectively. Example cross-sections through the image stacks were stored as CCM images.

Preparation: chemically fixed tissue
Imaging mode: confocal microscopy

• Cell Image Library Group ID: 18052

• Sprague-Dawley rat

• Atrial cells

• data
• image

• No linguistic content; Not applicable

Samplenumber: 48351

Doi: https://doi.org/10.6075/J02B8Z5C

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL48351

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2022-08-12