{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1441944000},"Data_type":{"Still_image":false,"Z_stack":false,"Video":false,"Time_series":true},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"48351.zip","Size":127957742,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"48351.tif","Size":885700000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"48351.jpg","Size":680064,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"TECHNICALDETAILS":{"free_text":"CCM images were from fixed rodent tissue preparations acquired using established methods (doi: 10.1161\/circimaging.112.000121). In short, rodent hearts were isolated and Langendorff-perfused with 4% paraformaldehyde. Tissue from the atrial working myocardium, sinoatrial node, and atrioventricular node were dissected from the hearts and fluorescently labeled with wheat germ agglutinin conjugated to CF488A (29022-1; Biotium, Hayward, CA; 1:25). Fluorescently labeled tissue preparations were imaged using a conventional laser-scanning confocal microscope (Zeiss LSM5 Duo; Zeiss, Jena, Germany) with a 40x oil immersion lens (NA 1.3). The fluorescent signal was captured using an Argon\/2 ion laser (488 nm excitation) and a bandpass filter (505 to 555 nm emission). Three-dimensional image stacks were acquired at a spatial resolution (xyz dimensions), field of view (xy) and a z-scan range of 0.2x0.2x0.2 μm, 204.8x204.8 μm and 50 μm, respectively. Example cross-sections through the image stacks were stored as CCM images."},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"ATTRIBUTION":{"Contributors":["Frank Roels"]},"DIMENSION":[{"Space":{"Pixel_size":{"unit":"microns","value":204.8},"axis":"X"}},{"Space":{"Pixel_size":{"unit":"microns","value":204.8},"axis":"Y"}},{"Space":{"Pixel_size":{"unit":"microns","value":50},"axis":"Z"}}],"IMAGEDESCRIPTION":{"free_text":"Images are acquired from fixed and living heart tissue using fiber-optics and laser-scanning confocal microscopy, respectively. Three sets of images denoted as CCM, FCMtopical, FCMcarrier were stored for subsequent analyses."},"NCBIORGANISMALCLASSIFICATION":{"free_text":"Sprague-Dawley rat"},"PREPARATION":{"onto_name":"chemically fixed tissue","onto_id":"FBbi:00000002"},"CELLULARCOMPONENT":{"free_text":"atrial cells"},"GROUP_ID":"18052"}},"Citation":{"Title":"Frank Roels (2015) CIL:48351, Sprague-Dawley rat. CIL. Dataset","ARK":"ark:\/b7295\/w9cil48351","DOI":"doi:10.7295\/W9CIL48351"}}}