Hoshijima, Masahiko; Hayashi, Takeharu (2021). CIL:40005, Mus musculus, regular cardiac myocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0FJ2GXN

Single computed slice from a tomographic reconstruction of a 500 nm section through the middle free wall of a cardiac myocyte from the left ventricle of an adult mouse (6-8 months of age), stained with potassium ferrocyanide to increase the contrast of membrane systems. For more information see: Hayashi, T., Martone, M. E., Yu, Z., Thor, A., Doi, M., Holst, M., Ellisman, M. H. and Hoshijima, M., Three-dimensional electron microscopy reveals new details of membrane systems for calcium signaling in the heart. J. Cell Science, 2009 Apr 1;122(Pt 7):1005-13. PMID: 19295127. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

• 2021

• Hayashi, Takeharu
• Hoshijima, Masahiko

Tissue was fixed with 2% paraformaldehyde and 2% glutaraldehyde in 0.15M sodium cacodylate, blocked into small pieces, post-fixed for 1-3 hours, washed 3x10min in 0.15M sodium cacodylate, then incubated in: 0.8% potassium ferrocyanide and 2% osmium tetroxide in 0.15M sodium cacodylate overnight at room temperature. Following another set of washes 2 hours-overnight in 0.15M sodium cacodylate tissue was stained en bloc with 1% uranyl acetate 30min, followed by a brief wash in DDH2O and standard dehydration and embedding. Images were gathered using a JEOL 4000, magnification of 15000.0, accelerating voltage, 400.0 kV.

Preparation: formaldehyde fixed tissue; glutaraldehyde fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Parameter imaged: electron density
Visualization methods: uranyl salt
Data qualification: Processed

• Mus musculus

• Intercalated disc
• Mitochondrion
• Regular cardiac myocyte
• Sarcomere
• T-tubule

• data
• image

• No linguistic content; Not applicable

Samplenumber: 40005

Doi: https://doi.org/10.6075/J0FJ2GXN

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40005

Previous version

• Item in Cell Centered Database, UC San Diego Library Digital Collections: https://doi.org/10.6075/J03B5ZWZ

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2022-08-12