{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1340683200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"40005.tif","Size":800000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"40005.jpg","Size":60115,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"CCDB 3603","Href":"http:\/\/ccdb.ucsd.edu\/sand\/main?event=displayAll&mpid=3603"}],"Contributors":["Masahiko Hoshijima"," Takeharu Hayashi"]},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Single computed slice from a tomographic reconstruction of a 500 nm section through the middle free wall of a cardiac myocyte from the left ventricle of an adult mouse (6-8 months of age), stained with potassium ferrocyanide to increase the contrast of membrane systems.  For more information see: Hayashi, T., Martone, M. E., Yu, Z., Thor, A., Doi, M., Holst, M., Ellisman, M. H. and Hoshijima, M., Three-dimensional electron microscopy reveals new details of membrane systems for calcium signaling in the heart. J. Cell Science, 2009 Apr 1;122(Pt 7):1005-13. PMID: 19295127.  This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"T-tubule","onto_id":"GO:0030315"},{"onto_name":"sarcomere","onto_id":"GO:0030017"},{"onto_name":"mitochondrion","onto_id":"GO:0005739"},{"onto_name":"intercalated disc","onto_id":"GO:0014704"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"TECHNICALDETAILS":{"free_text":"Tissue was fixed with 2% paraformaldehyde and 2% glutaraldehyde in 0.15M sodium cacodylate, blocked into small pieces, post-fixed for 1-3 hours, washed 3x10min in 0.15M sodium cacodylate, then incubated in: 0.8% potassium ferrocyanide and 2% osmium tetroxide in 0.15M sodium cacodylate overnight at room temperature.  Following another set of washes 2 hours-overnight in 0.15M sodium cacodylate tissue was stained en bloc with 1% uranyl acetate 30min, followed by a brief wash in DDH2O and standard dehydration and embedding.  Images were gathered using a JEOL 4000, magnification of 15000.0, accelerating voltage, 400.0 kV."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"IMAGINGMODE":{"onto_id":"FBbi:00000623"},"DIMENSION":[{"Space":{"Image_size":512,"axis":"X"}},{"Space":{"Image_size":512,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"}],"VISUALIZATIONMETHODS":{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},"CELLTYPE":{"onto_name":"regular cardiac myocyte","onto_id":"CL:0002098"}}},"Citation":{"Title":"Masahiko Hoshijima,  Takeharu Hayashi (2012) CIL:40005, Mus musculus, regular cardiac myocyte. CIL. Dataset","ARK":"ark:\/b7295\/w9cil40005","DOI":"doi:10.7295\/W9CIL40005"}}}