CIL:7429, Mus musculus, fibroblast, primary cell line cell
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- Cite This Work
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Thieverssen, Ingo; Waterman, Clare M. (2021). CIL:7429, Mus musculus, fibroblast, primary cell line cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0TX3DDP
- Description
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Image shows (actin polymerization at) free barbed ends and F-actin in the lamellipodium of a migrating primary murine embryonic fibroblast.
Cell has been mildly permeabilized using saponin during a 2min incubation in cytoskeleton buffer containing X-rhodamine labeled G-actin. Intracellular X-rhodamine actin polymerized into filaments at the very leading edge of the lamellipodium, resulting in the narrow red band along the tip of the lamelipodium. Lamellipodial F-actin was labeled with Alexa488-phalloidin using glutaraldehyde fixation subsequently to the X-rhodamine actin incubation.
The micrograph visualizes that the actin monomer incorporation into the lamellipodial actin filament structure is restricted to the front of the lamellipodium. This enables a directed extension of the branched actin network towards the plasmamembrane, which pushes the membrane forward thereby creating cell protrusion.
Wide field epifluorescence image taken on using a 100X 1.49 NA lens, Semrock DA/FI/TR/Cy5-4x4M-B Sedat filter set, and an Orca II with 500 ms exposure. - Date Issued
- 2021
- Researchers
- Technical Details
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Preparation: glutaraldehyde fixed tissue; saponin permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: widefield illumination; fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Alexa Fluor 488; X-Rhodamine; phalloidin
Processing history: unprocessed raw data - Scientific Name
- Anatomy
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- No linguistic content; Not applicable
- Identifier
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Samplenumber: 7429
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7429
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12