Thieverssen, Ingo; Waterman, Clare M. (2021). CIL:7429, Mus musculus, fibroblast, primary cell line cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0TX3DDP

Image shows (actin polymerization at) free barbed ends and F-actin in the lamellipodium of a migrating primary murine embryonic fibroblast.
Cell has been mildly permeabilized using saponin during a 2min incubation in cytoskeleton buffer containing X-rhodamine labeled G-actin. Intracellular X-rhodamine actin polymerized into filaments at the very leading edge of the lamellipodium, resulting in the narrow red band along the tip of the lamelipodium. Lamellipodial F-actin was labeled with Alexa488-phalloidin using glutaraldehyde fixation subsequently to the X-rhodamine actin incubation.
The micrograph visualizes that the actin monomer incorporation into the lamellipodial actin filament structure is restricted to the front of the lamellipodium. This enables a directed extension of the branched actin network towards the plasmamembrane, which pushes the membrane forward thereby creating cell protrusion.

Wide field epifluorescence image taken on using a 100X 1.49 NA lens, Semrock DA/FI/TR/Cy5-4x4M-B Sedat filter set, and an Orca II with 500 ms exposure.

• 2021

• Thieverssen, Ingo
• Waterman, Clare M.

Preparation: glutaraldehyde fixed tissue; saponin permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: widefield illumination; fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Alexa Fluor 488; X-Rhodamine; phalloidin
Processing history: unprocessed raw data

• Mus musculus

• Actin cytoskeleton
• Fibroblast
• Primary cell line cell

• Actin binding
• Actin filament polymerization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7429

Doi: https://doi.org/10.6075/J0TX3DDP

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7429

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2022-08-12