Palade, George E. (2021). CIL:37200, Cavia porcellus, pancreas. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0FJ2FFZ

Transmission electron micrograph of guinea pig pancreas mitochondria and lipid droplets. The use of osmium tetroxide as a fixative for electron microscopy was first described by Dr. Palade at the Rockefeller. Another major advance in fixation for electron microscopy was done at Yale by David Sabatini who described the use of glutaraldehyde which not only preserved ultrastructure, but enzymatic activities which opened up the possibility of EM localization of enzymatic function in cells. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.

• 1968-05-15

• 2021

• Palade, George E.

References:
Palade, G.E. 1952. J. Exp. Med. 95:285-298.
Palade, G.E. 1952. Anat. Rec. 114:427-451.
Sabatini, D.D, K. Bensch and R.J. Barrnett. 1963. J. Cell. Bio. 17:19-58.
Original 3.25 in. x 4 in. lantern slides were scanned at 600dpi. Original magnification X12,100.

Preparation: osmium tetroxide fixed tissue
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain

• Cell Image Library Group ID: 10000

• Cavia porcellus

• Lipid droplets
• Mitochondrial crista
• Mitochondrion
• Pancreas

• data
• image

• No linguistic content; Not applicable

Samplenumber: 37200

Doi: https://doi.org/10.6075/J0FJ2FFZ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL37200

Other resource

• George E. Palade EM Slide Collection: https://library.medicine.yale.edu/find/palade

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2023-08-17