{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1319860800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"37200.jpg","Size":5820958,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"37200.tif","Size":56800000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"37200.zip","Size":56731478,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"DATE":["05\/15\/1968"],"URLs":[{"Label":"George E. Palade EM Slide Collection","Href":"http:\/\/cushing.med.yale.edu\/gsdl\/cgi-bin\/library?c=palade&a=d&d=DpaladeMitoJ"}],"Contributors":["George E. Palade"]},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Transmission electron micrograph of guinea pig pancreas mitochondria and lipid droplets.  The use of osmium tetroxide as a fixative for electron microscopy was first described by Dr. Palade at the Rockefeller. Another major advance in fixation for electron microscopy was done at Yale by David Sabatini who described the use of glutaraldehyde which not only preserved ultrastructure, but enzymatic activities which opened up the possibility of EM localization of enzymatic function in cells.  Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"mitochondrion","onto_id":"GO:0005739"},{"free_text":"lipid droplets"},{"onto_name":"mitochondrial crista","onto_id":"GO:0030061"}],"RELATIONTOINTACTCELL":{"onto_name":"sectioned tissue","onto_id":"FBbi:00000026"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"10000","TECHNICALDETAILS":{"free_text":"References:\nPalade, G.E. 1952.  J. Exp. Med. 95:285-298.\nPalade, G.E. 1952. Anat. Rec. 114:427-451.\nSabatini, D.D, K. Bensch and R.J. Barrnett. 1963. J. Cell. Bio. 17:19-58. \nOriginal 3.25 in. x 4 in. lantern slides were scanned at 600dpi.  Original magnification X12,100."},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":6000,"axis":"X"}},{"Space":{"Image_size":4727,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Cavia porcellus","onto_id":"NCBITaxon:10141"},"PREPARATION":{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},"CELLTYPE":{"free_text":"pancreas"}}},"Citation":{"Title":"George E. Palade (2011) CIL:37200, Cavia porcellus, pancreas. CIL. Dataset","ARK":"ark:\/b7295\/w9cil37200","DOI":"doi:10.7295\/W9CIL37200"}}}