CIL:35065
CIL source metadata (JSON)
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- Collection
- Cite This Work
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Svitkina, Tatyana M.; Borisy, Gary G. (2021). CIL:35065. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0CN72KH
- Description
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Electron micrograph of keratocyte or fibroblast lamellipodial actin network after unprotected extraction. All examples demonstrate frequent branching of actin filaments. Image corresponds to a single panel from Figure 2d in J Cell Biol. 1999 May 31;145(5):1009-26. All of the panels from Figure 2d can be found as CIL 35060-35068.
- Date Issued
- 2021
- Researchers
- Methods
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Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).
- Technical Details
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Preparation: permeabilized tissue; glutaraldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: shadowing and plating; platinum replica
Processing history: unprocessed raw data - Series
- Anatomy
- Topics
Formats
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 35065
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35065
- J Cell Biol. 1999 May 31;145(5):1009-26. https://www.ncbi.nlm.nih.gov/pubmed/?term=10352018
Source data
Reference
- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12