{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1307073600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"35065.tif","Size":300000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"35065.jpg","Size":57886,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"35065.zip","Size":448198,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Biol. 1999 May 31;145(5):1009-26."],"Contributors":["Tatyana M. Svitkina","Gary G. Borisy"],"PUBMED":["10352018"]},"BIOLOGICALPROCESS":[{"free_text":"branching of actin filaments"},{"onto_name":"actin filament-based process","onto_id":"GO:0030029"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"optical path length gradient","onto_id":"FBbi:00000312"},"IMAGEDESCRIPTION":{"free_text":"Electron micrograph of keratocyte or fibroblast lamellipodial actin network after unprotected extraction.  All examples demonstrate frequent branching of actin filaments.  Image corresponds to a single panel from Figure 2d in J Cell Biol. 1999 May 31;145(5):1009-26.  All of the panels from Figure 2d can be found as CIL 35060-35068."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"actin cytoskeleton","onto_id":"GO:0015629"},"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"boundaries between regions with different refractive index","onto_id":"FBbi:00000599"},"GROUP_ID":"9875","MOLECULARFUNCTION":{"onto_name":"structural constituent of cytoskeleton","onto_id":"GO:0005200"},"TECHNICALDETAILS":{"free_text":"Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998)."},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":453,"axis":"X"}},{"Space":{"Image_size":457,"axis":"Y"}}],"PREPARATION":[{"onto_name":"permeabilized tissue","onto_id":"FBbi:00000093"},{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"}],"VISUALIZATIONMETHODS":[{"onto_name":"shadowing and plating","onto_id":"FBbi:00000398"},{"free_text":"platinum replica"}]}},"Citation":{"Title":"Tatyana M. Svitkina, Gary G. Borisy (2011) CIL:35065. CIL. Dataset","ARK":"ark:\/b7295\/w9cil35065","DOI":"doi:10.7295\/W9CIL35065"}}}