Gill, David J.; Chia, Joanne; Senewiratne, Jamie; Bard, Frederic (2021). CIL:13577, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0MC8XQF

Co-expression of wild-type Arf1-GFP (Arf1(WT)-GFP) (gray) and inactive Src (SrcK-DsRed) (red) in Src-deficient SYF fibroblasts does not induce trafficking of Helix Pomatia Lectin (HPL) (green) from the Golgi. HPL binds various glycans but the Tn antigen in particular. The Tn antigen refers to terminal α-linked N-acetyl galactosamine residues (GalNAc) linked to Ser or Thr residues. HPL indicates distribution of GalNac-Ts. Inactive Src is highly enriched at the Golgi. Cells were fixed for 10 min (4% paraformaldehyde) and permeabilized (0.2% Triton X-100) prior to staining with Hoechst (blue) and Alexa 647-conjugated-HPL. Cells were mounted onto glass slides using FluorSave (Merck) and imaged at room temperature using an inverted FluoView confocal microscope (model IX81; Olympus) with either a 60x objective (U Plan Super Apochromatic, UPLSAPO; NA 1.35) or 100x objective (UPLSAPO; NA 1.40) using Immersol oil. Microscope coupled with a CCD camera (model FVII). Images were acquired and processed using Olympus FV10-ASW software. Image corresponds to Fig 7F in J Cell Biol. 189: 843-858. 2010. Images in Fig 7 include CIL#s 13571, 13572, 13573, 13574, 13575, 13576, 13577.

• 2021

• Bard, Frederic
• Chia, Joanne
• Gill, David J.
• Senewiratne, Jamie

Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence microscopy
Source of contrast: distribution of a specific protein; distribution of epitope; compartmentalization of stain or label
Visualization methods: Alexa Fluor 647; Alexa Fluor 594; EGFP; primary antibody plus labeled secondary antibody; Helix pomatia agglutinin; Hoechst
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 6490

• Homo sapiens

• Cervical carcinoma
• Epithelial cell
• Golgi stack
• Integral to membrane
• Nucleus

• COPI coating of Golgi vesicle
• Golgi organization
• GTPase activity
• HeLa
• Protein binding
• Retrograde vesicle-mediated transport, Golgi to ER
• Small GTPase mediated signal transduction

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13577

Doi: https://doi.org/10.6075/J0MC8XQF

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13577

Reference

• J Cell Biol. 189: 843-858. 2010. https://www.ncbi.nlm.nih.gov/pubmed/?term=20498016

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

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2022-08-12