{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1301284800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"13577.zip","Size":8402143,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"13577.jpg","Size":77223,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"13577.tif","Size":8500000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/2860\/"}],"PUBLISHED":["J Cell Biol. 189: 843-858. 2010."],"Contributors":["David J. Gill","Joanne Chia","Jamie Senewiratne","Frederic Bard"],"PUBMED":["PMID: 20498016"]},"BIOLOGICALPROCESS":[{"onto_name":"Golgi organization","onto_id":"GO:0007030"},{"onto_name":"COPI coating of Golgi vesicle","onto_id":"GO:0048205"},{"onto_name":"retrograde vesicle-mediated transport, Golgi to ER","onto_id":"GO:0006890"},{"onto_name":"small GTPase mediated signal transduction","onto_id":"GO:0007264"}],"CELLLINE":{"onto_name":"HeLa","onto_id":"MCC:0000219"},"PARAMETERIMAGED":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"IMAGEDESCRIPTION":{"free_text":"Co-expression of wild-type Arf1-GFP (Arf1(WT)-GFP) (gray) and inactive Src (SrcK-DsRed) (red) in Src-deficient SYF fibroblasts does not induce trafficking of  Helix Pomatia Lectin (HPL) (green) from the Golgi. HPL binds various glycans but the Tn antigen in particular. The Tn antigen refers to terminal α-linked N-acetyl galactosamine residues (GalNAc) linked to Ser or Thr residues. HPL indicates distribution of GalNac-Ts. Inactive Src is highly enriched at the Golgi. Cells were fixed for 10 min (4% paraformaldehyde) and permeabilized (0.2% Triton X-100) prior to staining with Hoechst (blue) and Alexa 647-conjugated-HPL. Cells were mounted onto glass slides using FluorSave (Merck) and imaged at room temperature using an inverted FluoView confocal microscope (model IX81; Olympus) with either a 60x objective (U Plan Super Apochromatic, UPLSAPO; NA 1.35) or 100x objective (UPLSAPO; NA 1.40) using Immersol oil. Microscope coupled with a CCD camera (model FVII). Images were acquired and processed using Olympus FV10-ASW software. Image corresponds to Fig 7F in J Cell Biol. 189: 843-858. 2010. Images in Fig 7 include CIL#s 13571, 13572, 13573, 13574, 13575, 13576, 13577."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"Golgi stack","onto_id":"GO:0005795"},{"onto_name":"integral to membrane","onto_id":"GO:0016021"},{"onto_name":"nucleus","onto_id":"GO:0005634"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":[{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"},{"onto_name":"compartmentalization of stain or label","onto_id":"FBbi:00000604"}],"GROUP_ID":"6490","MOLECULARFUNCTION":[{"onto_name":"GTPase activity","onto_id":"GO:0003924"},{"onto_name":"protein binding","onto_id":"GO:0005515"}],"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"single-spot confocal microscopy","onto_id":"FBbi:00000252"},"DIMENSION":[{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.207},"axis":"X"}},{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.207},"axis":"Y"}},{"Space":{"Image_size":1,"Pixel_size":{"unit":"microns","value":13.95},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"detergent permeabilized","onto_id":"FBbi:00000262"}],"VISUALIZATIONMETHODS":[{"onto_name":"Alexa Fluor 647","onto_id":"FBbi:00000447"},{"onto_name":"Alexa Fluor 594","onto_id":"FBbi:00000444"},{"onto_name":"EGFP","onto_id":"FBbi:00000082"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"onto_name":"Helix pomatia agglutinin","onto_id":"FBbi:00000429"},{"free_text":"Hoechst"}],"CELLTYPE":[{"onto_name":"epithelial cell","onto_id":"CL:0000066"},{"free_text":"cervical carcinoma"}]}},"Citation":{"Title":"David J. Gill, Joanne Chia, Jamie Senewiratne, Frederic Bard (2011) CIL:13577, Homo sapiens, epithelial cell, cervical carcinoma. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13577","DOI":"doi:10.7295\/W9CIL13577"}}}