Ladinsky, Mark S.; Wu, Christine C.; McIntosh, Shane; McIntosh, J. Richard; Howell, Kathryn E. (2021). CIL:24777, Rattus norvegicus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0959GQK

This image is one from a set of 40nm thick serial sections through part of a Golgi ribbon from a normal rat kidney cell, generated using transmission electron microscopy following incubation at 15°C to block transport out of the Golgi complex. Following temperature block, large bulging domains appear on the three trans-most cisternae. The images in this set were used in 3-dimensional reconstructions of the Golgi apparatus. For more information see: Ladinsky et al. (2002) Structure of the Golgi and distribution of reporter molecules at 20°C reveals the complexity of the exit compartments, Mol Biol Cell 13:2810-2825. The other images from this serial set are included in this image group.

• 2021

• Howell, Kathryn E.
• Ladinsky, Mark S.
• McIntosh, J. Richard
• McIntosh, Shane
• Wu, Christine C.

Cells were grown on 100-mesh gold EM grids, and maintained at 15°C for 4 hours before plunge freezing in liquid nitrogen (BalTec HPM-010), followed by freeze-substitution (1% glutaraldehyde, 0.1% tannic acid in acetone, replaced with 4% osmium tetroxide and 0.01% uranyl acetate), then embedded in Epon-Araldite and sectioned at 40nm (UltraCut-UCT, Leica). Sections were transferred to formvar-coated copper-rhodium slot grids (EMS) and stained with 2% aqueous uranyl acetate and Reynold's lead citrate. Images were acquired at a magnification of 13,000X with an FEI Tecnai TF20 transmission EM. For additional details refer to: Mol Biol Cell 13:2810-2825.

Preparation: tissue in epoxy resin embedment; osmium tetroxide fixed tissue; glutaraldehyde fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: uranyl salt; lead salt
Processing history: unprocessed raw data
Data qualification: Raw

• Cell Image Library Group ID: 8573

• Rattus norvegicus

• Epithelial cell
• Golgi apparatus
• Golgi cisterna

• Golgi organization
• Golgi vesicle budding
• Golgi vesicle transport
• NRK-52E

• data
• image

• No linguistic content; Not applicable

Samplenumber: 24777

Doi: https://doi.org/10.6075/J0959GQK

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL24777

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12