{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1308628800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"24777.jpg","Size":380990,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"24777.tif","Size":1200000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"Contributors":["Mark S. Ladinsky","Christine C. Wu","Shane McIntosh","J. Richard McIntosh","Kathryn E. Howell"]},"BIOLOGICALPROCESS":[{"onto_name":"Golgi organization","onto_id":"GO:0007030"},{"onto_name":"Golgi vesicle budding","onto_id":"GO:0048194"},{"onto_name":"Golgi vesicle transport","onto_id":"GO:0048193"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"NRK-52E","onto_id":"MCC:0000366"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"This image is one from a set of 40nm thick serial sections through part of a Golgi ribbon from a normal rat kidney cell, generated using transmission electron microscopy following incubation at 15°C to block transport out of the Golgi complex.  Following temperature block, large bulging domains appear on the three trans-most cisternae.  The images in this set were used in 3-dimensional reconstructions of the Golgi apparatus.  For more information see: Ladinsky et al. (2002) Structure of the Golgi and distribution of reporter molecules at 20°C reveals the complexity of the exit compartments, Mol Biol Cell 13:2810-2825. The other images from this serial set are included in this image group."},"ITEMTYPE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"CELLULARCOMPONENT":[{"onto_name":"Golgi apparatus","onto_id":"GO:0005794"},{"onto_name":"Golgi cisterna","onto_id":"GO:0031985"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"8573","TECHNICALDETAILS":{"free_text":"Cells were grown on 100-mesh gold EM grids, and maintained at 15°C for 4 hours before plunge freezing in liquid nitrogen (BalTec HPM-010), followed by freeze-substitution (1% glutaraldehyde, 0.1% tannic acid in acetone, replaced with 4% osmium tetroxide and 0.01% uranyl acetate), then embedded in Epon-Araldite and sectioned at 40nm (UltraCut-UCT, Leica).  Sections were transferred to formvar-coated copper-rhodium slot grids (EMS) and stained with 2% aqueous uranyl acetate and Reynold's lead citrate.  Images were acquired at a magnification of 13,000X with an FEI Tecnai TF20 transmission EM. For additional details refer to: Mol Biol Cell 13:2810-2825."},"DATAQUALIFICATION":{"free_text":"RAW"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"},{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"}],"DIMENSION":[{"Space":{"Image_size":1078,"Pixel_size":{"unit":"microns","value":0.0118},"axis":"X"}},{"Space":{"Image_size":1031,"Pixel_size":{"unit":"microns","value":0.0118},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Rattus norvegicus","onto_id":"NCBITaxon:10116"},"PREPARATION":[{"onto_id":"FBbi:00000621"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"}],"VISUALIZATIONMETHODS":[{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":{"onto_name":"epithelial cell","onto_id":"CL:0000066"}}},"Citation":{"Title":"Mark S. Ladinsky, Christine C. Wu, Shane McIntosh, J. Richard McIntosh, Kathryn E. Howell (2011) CIL:24777, Rattus norvegicus, epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil24777","DOI":"doi:10.7295\/W9CIL24777"}}}