Vicente-Manzanares, Miguel; Newell-Litwa, Karen; Bachir, Alexia I.; Whitmore, Leanna A.; Horwitz, Alan Rick (2021). CIL:25846, Cricetulus griseus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0X9295N

Time-lapse of a CHO.K1 cell cotransfected with very low levels of GFP-MIIA (myosin IIA, green) and paxillin (magenta). Yellow arrowheads mark paxillin-containing adhesions that disassemble concomitantly to their MIIA-decorated bundle (marked with arrows) as the movie progresses. Total internal reflection fluorescence (TIRF) images were acquired on an inverted microscope (IX70; Olympus) with a 1.45 NA (oil) Plan-Apochromat 60× TIRFM objective lens. Images were acquired with a charge-coupled device (CCD) camera (Retiga Exi; Qimaging). Video corresponds to Fig 5C and Video 5 from J Cell Biol 2011 193:381-396.

• 2021

• Bachir, Alexia I.
• Horwitz, Alan Rick
• Newell-Litwa, Karen
• Vicente-Manzanares, Miguel
• Whitmore, Leanna A.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: TIRF
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP; mCherryFP
Processing history: unproc

• Cell Image Library Group ID: 7724

• Cricetulus griseus

• Epithelial cell
• Focal adhesion
• Myosin II complex

• CHO-K1
• Focal adhesion disassembly

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 25846

Doi: https://doi.org/10.6075/J0X9295N

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL25846

Reference

• J Cell Biol 2011 193:381-396 https://www.ncbi.nlm.nih.gov/pubmed/?term=21482721

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2022-08-12