{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1303963200},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Mp4","File_path":"25846_web.mp4","Size":514608,"Mime_type":"video\/mp4"},{"File_type":"Zip","File_path":"25846.zip","Size":1179776,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"25846.jpg","Size":6213,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Biol 2011 193:381-396"],"Contributors":["Miguel Vicente-Manzanares","Karen Newell-Litwa","Alexia I. Bachir","Leanna A. Whitmore","Alan Rick Horwitz"],"PUBMED":["21482721"]},"BIOLOGICALPROCESS":{"free_text":"focal adhesion disassembly"},"PROCESSINGHISTORY":{"free_text":"unproc"},"CELLLINE":{"onto_name":"CHO-K1","onto_id":"MCC:0000103"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Time-lapse of a CHO.K1 cell cotransfected with very low levels of GFP-MIIA (myosin IIA, green) and paxillin (magenta). Yellow arrowheads mark paxillin-containing adhesions that disassemble concomitantly to their MIIA-decorated bundle (marked with arrows) as the movie progresses. Total internal reflection fluorescence (TIRF) images were acquired on an inverted microscope (IX70; Olympus) with a 1.45 NA (oil) Plan-Apochromat 60× TIRFM objective lens.  Images were acquired with a charge-coupled device (CCD) camera (Retiga Exi; Qimaging).  Video corresponds to Fig 5C and Video 5 from J Cell Biol 2011 193:381-396."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"focal adhesion","onto_id":"GO:0005925"},{"onto_name":"myosin II complex","onto_id":"GO:0016460"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"7724","TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"free_text":"TIRF"},"DIMENSION":[{"Space":{"Image_size":248,"axis":"X"}},{"Space":{"Image_size":258,"axis":"Y"}},{"Time":{"unit":"seconds","value":5}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Cricetulus griseus","onto_id":"NCBITaxon:10029"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":[{"onto_name":"EGFP","onto_id":"FBbi:00000082"},{"onto_name":"mCherryFP","onto_id":"FBbi:00000525"}],"CELLTYPE":{"onto_name":"epithelial cell","onto_id":"CL:0000066"}}},"Citation":{"Title":"Miguel Vicente-Manzanares, Karen Newell-Litwa, Alexia I. Bachir, Leanna A. Whitmore, Alan Rick Horwitz (2011) CIL:25846, Cricetulus griseus, epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil25846","DOI":"doi:10.7295\/W9CIL25846"}}}