von Dassow, George; Verbrugghe, Koen J.C.; Miller, Ann L.; Sider, Jenny R.; Bement, William M. (2021). CIL:15788, Strongylocentrotus purpuratus. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0RX99VX

16-cell purple urchin embryo expressing three tandem GFPs fused to the ensconsin microtubule-binding domain (EMTB-3G; Faire et al., 1999). The embryo was treated with 20 µM trichostatin A (TSA), an inhibitor of HDAC6 (a tubulin deacetylase) that selectively disrupts dynamic microtubules in mammalian cells, at time 00:00. Within minutes of TSA addition in metaphase, asters were reduced to nearly nothing (04:00). Despite reduction in spindle length, cells enter anaphase (07:20) and complete cytokinesis (11:20–16:10). No microtubules connecting the mitotic apparatus to the cortex are visible. Embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens at room temperature. Corresponds to Figure 4A and first segment of video 4 in J Cell Biol. 2009 Dec 14;187(6):831-45.

• 2021

• Bement, William M.
• Miller, Ann L.
• Sider, Jenny R.
• Verbrugghe, Koen J.C.
• Von Dassow, George

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data

• Cell Image Library Group ID: 6184

• Strongylocentrotus purpuratus

• Astral microtubule
• Ensconsin
• Microtubule
• Spindle

• Cytokinesis after mitosis
• Embryo
• Mitotic anaphase
• Mitotic metaphase
• Trichostatin A treatment

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 15788

Doi: https://doi.org/10.6075/J0RX99VX

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL15788

Reference

• J Cell Biol. 2009 Dec 14;187(6):831-45. https://www.ncbi.nlm.nih.gov/pubmed/?term=20008563

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2022-08-12